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  • 1 Department of Biotechnology, West Bengal University of Technology, BF-142, Saltlake, Kolkata, 700 064 India
  • 2 Department of Zoology, Kalyani University, Kalyani, India
  • 3 Information Technology, B. P. Poddar Institute of Management and Technology, West Bengal, India
  • 4 Army Referral Hospital, New Delhi, India
  • 5 Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064 India
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Abstract  

The paper aims to investigate cytogenetic and apoptotic responses of γ-irradiation in a radio-resistant cell strain designated as M5. Induced micronuclei, chromosomal aberrations, nuclear fragmentation and nucleosomal ladders by γ-irradiation were less at equal doses in M5 cells in comparison with that obtained in the parental Chinese hamster V79 cells. However, at equal survival, there were no differences in the end points studied. Results indicate that the residual damages that lead to reproductive cell death also resulted in the cytogenetic and apoptotic responses. We speculate that the repair efficiency in M5 cells was more efficient and increased DNA repair could be the cause of radiation resistance observed in M5 cells.