DNA comet assay: A simple screening technique for identification of some irradiated foods

A. Khan; H. Khan

doi:10.1007/s10967-007-7068-4

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 275: Issue 2

DNA comet assay: A simple screening technique for identification of some irradiated foods

Authors:

A. Khan

A. Khan University of Peshawar Radiation Chemistry Laboratory, National Center of Excellence in Physical Chemistry Peshawar 25120 Pakistan

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H. Khan

H. Khan University of Peshawar Radiation Chemistry Laboratory, National Center of Excellence in Physical Chemistry Peshawar 25120 Pakistan

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Pages:: 337–342

Online Publication Date:: 27 Sep 2007

Publication Date:: 01 Feb 2008

Article Category:: Research Article

DOI:: https://doi.org/10.1007/s10967-007-7068-4

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Abstract

DNA Comet Assay method was carried out to detect irradiation treatment of some foods like meat, spices, beans and lentils. The fresh meat of cow and duck were irradiated up to radiation doses of 3 kGy, the spices (cardamoms and cumin black) were irradiated to radiation doses of 5, 10, 15 and 20 kGy while the beans (black beans and white beans) and lentils (red and green lentils) were irradiated to 0.5 and 1 kGy. All the foods were then analyzed for radiation treatment using simple microgel electrophoresis of single cells or nuclei (DNA Comet Assay). Sedimentation, lysis and staining times were adjusted to get optimized conditions for correct and easy analysis of each food. Using these optimized conditions, it was found out that radiation damaged DNA showed comets in case of irradiated food samples, whereas in non-treated food samples, round or conical spots of stained DNA were visible. Shape, length and intensity of these comets were also radiation dose dependent. Screening of unirradiated and irradiated samples by Comet Assay was successful in the case of all the foods under consideration under the optimized conditions of assay. Therefore, for different kinds of irradiated foods studied in the present study, the DNA Comet Assay can be used as a rapid, simple and inexpensive screening test.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)