DNA Comet Assay method was carried out to detect irradiation treatment of some foods like meat, spices, beans and lentils.
The fresh meat of cow and duck were irradiated up to radiation doses of 3 kGy, the spices (cardamoms and cumin black) were
irradiated to radiation doses of 5, 10, 15 and 20 kGy while the beans (black beans and white beans) and lentils (red and green
lentils) were irradiated to 0.5 and 1 kGy. All the foods were then analyzed for radiation treatment using simple microgel
electrophoresis of single cells or nuclei (DNA Comet Assay). Sedimentation, lysis and staining times were adjusted to get
optimized conditions for correct and easy analysis of each food. Using these optimized conditions, it was found out that radiation
damaged DNA showed comets in case of irradiated food samples, whereas in non-treated food samples, round or conical spots
of stained DNA were visible. Shape, length and intensity of these comets were also radiation dose dependent. Screening of
unirradiated and irradiated samples by Comet Assay was successful in the case of all the foods under consideration under the
optimized conditions of assay. Therefore, for different kinds of irradiated foods studied in the present study, the DNA Comet
Assay can be used as a rapid, simple and inexpensive screening test.