Authors:
J. Baptista Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP) Av. Professor Lineu Prestes 2242 05508-000 São Paulo, SP Brasil

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D. Vieira

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A. Galisteo-Júnior

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P. Caproni Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP) Av. Professor Lineu Prestes 2242 05508-000 São Paulo, SP Brasil

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M. Casare Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP) Av. Professor Lineu Prestes 2242 05508-000 São Paulo, SP Brasil

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H. de Andrade-Júnior

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P. Spencer Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP) Av. Professor Lineu Prestes 2242 05508-000 São Paulo, SP Brasil

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N. Nascimento Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP) Av. Professor Lineu Prestes 2242 05508-000 São Paulo, SP Brasil

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Abstract  

We investigated the immunological behavior of BTHX-1, before and after irradiation. SDS-PAGE showed that BTHX-1 irradiated in the presence of NaNO3, had its structure preserved. Animals’ plasma immunized with native BTHX-1 had high IgG1 titers. The irradiated protein induced high titers of IgG2b. When the toxin was irradiated with t-butanol, there was a slight decrease in the production of IgG2b. Real-time PCR showed that both the IL-2 as for IL4 was more expression from the cells of the animals immunized with BTHX-1 irradiated. These results indicate that irradiation of proteins leads to significant structural modifications.

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)