Copper-64 was produced by the 64Ni(p, n)64Cu reaction using enriched 64NiO target. We investigated and compared the production yield of 64Cu for proton beams of various energies by using a thick target. Enriched 64Ni was recovered with high yield by simple procedures. Imaging studies using positron emission tomography (PET) and positron
emitting tracer imaging system (PETIS) were performed. We obtained clear images in PET and PETIS studies. The results of this
study indicate that 64Cu can be utilized as a biomedical tracer for the molecular imaging both in animals and plants.