Authors:
V. Lungu Horia Hulubei National Institute for Physics and Nuclear Engineering Magurele, Bucharest Romania

Search for other papers by V. Lungu in
Current site
Google Scholar
PubMed
Close
,
D. Chiper Horia Hulubei National Institute for Physics and Nuclear Engineering Magurele, Bucharest Romania

Search for other papers by D. Chiper in
Current site
Google Scholar
PubMed
Close
,
R. Anghel Alexandru Trestioreanu Institute of Oncology Bucharest Romania

Search for other papers by R. Anghel in
Current site
Google Scholar
PubMed
Close
,
C. Postolache Horia Hulubei National Institute for Physics and Nuclear Engineering Magurele, Bucharest Romania

Search for other papers by C. Postolache in
Current site
Google Scholar
PubMed
Close
,
L. Matei Horia Hulubei National Institute for Physics and Nuclear Engineering Magurele, Bucharest Romania

Search for other papers by L. Matei in
Current site
Google Scholar
PubMed
Close
,
C. Barna Horia Hulubei National Institute for Physics and Nuclear Engineering Magurele, Bucharest Romania

Search for other papers by C. Barna in
Current site
Google Scholar
PubMed
Close
, and
G. Bubueanu Horia Hulubei National Institute for Physics and Nuclear Engineering Magurele, Bucharest Romania

Search for other papers by G. Bubueanu in
Current site
Google Scholar
PubMed
Close
Restricted access

Abstract  

The aim of this study was to evaluate the effect of methyl donor biovectors as cocktail formulation in cancer therapy. Based on our previous results regarding the pharmacokinetic of [Methyl-14C] Choline and [Methyl-3H] S-Adenosyl-Methionine (SAM) in presence of Folic Acid and their properties in the regulation of transmethylation metabolism, a cocktail solution of SAM, Choline and Folic Acid was prepared taking into account the previosly established molar ratios. The studies regarding the therapeutic effect of cocktail solution were effectuated on RS1 tumor bearing animal models. For evaluation of tumour cell proliferation we used the [Methyl-3H]-Thymidine with 25 Ci/mmol specific activity. The biodistribution studies were perfomed on untreated and treated tumuor bearing animals. The animal lots dedicated for treatment with methyl donors cocktail were treated with 200 μL cocktail for 4 days. After finishing the treatment, both untreated and treated animal lots were injected with 150 μCi [Methyl-3H]-Thymidine. Biodistribution studies were performed at 2, 4 and 8 hours post injection. The biodistribution results showed that at tumor level the uptake of [Methyl-3H]Thymidine is higher in the untreated animals (0.78%ID/g at 2 h, 1.07%ID/g at 4 h and 1.35%ID/g at 8 h) than in treated animals (0.06%ID/g at 2 h, 0.11%ID/g at 4 h and 0.13%ID/g at 8 h). The results regarding the Homocysteine levels in investigated animals show a decreasing of Homocysteine concentration up to normal values for treated animals.

  • Collapse
  • Expand

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)