Preparation of 99mTc-metronidazole as a model for tumor imaging

Ismail Ibrahim

doi:10.1007/s10967-009-0040-8

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 281: Issue 3

Preparation of ^99mTc-metronidazole as a model for tumor imaging

Author:

Ismail Ibrahim

Ismail Ibrahim Atomic Energy Authority Hot Laboratories Center P.O. Box 13759 Cairo Egypt

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Pages:: 669–674

Online Publication Date:: 25 Sep 2009

Publication Date:: 01 Sep 2009

Article Category:: Research Article

DOI:: https://doi.org/10.1007/s10967-009-0040-8

Keywords:: Metronidazole; 99mTc; Labeling; Biodistribution; EAC

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Abstract

Metronidazole (MTNZ) is an antiprotozoa drug, could be labeled with the ^99mTc. MTZL could be used as an ideal vehicle to deliver radioactive decay energy of ^99mTc to the sites of tumor, thus facilitate tumor imaging. The process of labeling was done using tin chloride as reducing agent. The optimum conditions required to label 25 μg MTZL were 100 μg stannous chloride, 30 min reaction time, room temperature at pH 7–9 using 0.5 M phosphate buffer. The radiochemical purity of the labeled compound, at the above conditions, was determined using paper chromatography. The yield was about 93%. About 2.5 × l0⁶ of Ehrlich Ascites Carcinoma (EAC) was injected intrapritoneally (i.p) to produce ascites and intramuscularly (i.m) in the right thigh to produce solid tumor in female mice. Biodistribution studies were carried out by injecting solution of ^99mTc-MTZL in normal and tumor bearing mice. The uptake in ascites was over 5% of the injected dose per gram tissue body weight, at 4 h post injection and above 4% in solid tumor. These data revealed localization of the tracer in the tumor tissues with high percentage sufficient to use ⁹⁹ ^mTc MTZL as promising tool for diagnosis of tumor.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)