In this work, the authors investigated the immunological behavior of bothropstoxin-I (BTHX-1), before and after irradiation
process, and also the influence of scavengers substances on protein alterations induced by free radical production. Structural
modifications were investigated by SDS-PAGE in reducing or non-reducing conditions. In vitro cytotoxicity assay was performed
to test average toxic activities of BTHX-I. BALB/c Isogenic mice were immunized with irradiated or non-irradiated (native)
forms of BTHX-I and antibody titers and isotypes were determined by ELISA method. Expression of murine cytokines was analyzed
by using expression data obtained by quantitative real-time PCR (qPCR) assays. The results indicate that irradiation of proteins
leads to significant structural modifications, and also changes the cytokines profile during immunization process, regarding
a suitable approach to new immunogenic production.