Radiocharacterization of the 99mTc–rufloxacin complex and biological evaluation in Staphylococcus aureus infected rat model

Syed Shah; Muhammad Khan

doi:10.1007/s10967-010-0923-8

Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 288: Issue 2

Radiocharacterization of the ^99mTc–rufloxacin complex and biological evaluation in Staphylococcus aureus infected rat model

Authors:

Syed Shah

Syed Shah Nuclear Medicine Research Laboratory (NMRL), University of Peshawar, Peshawar, KPK Pakistan

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Muhammad Khan

Muhammad Khan Phyotopharmaceutical and Neutraceuticals Research Laboratory (PNRL), University of Peshawar, Peshawar, KPK Pakistan

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Pages:: 373–378

Online Publication Date:: 23 Dec 2010

Publication Date:: 01 May 2011

Article Category:: Research Article

DOI:: https://doi.org/10.1007/s10967-010-0923-8

Keywords:: Rufloxacin; 99mTc; Biodistribution; Staphylococcus aureus ; Infection

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Abstract

^99mTc–rufloxacin (^99mTc–RUN) complex was prepared by reaction of different amounts of reduced sodium pertechnetate with different amount of Rufloxacin (RUN) antibiotic for the in vivo scintigraphic localization of the Staphylococcus aureus (S. aureus) infectious foci in Male Wister Rats (MWR) model. The ^99mTc–RUN complex was radiochemically and biologically characterized in terms of radiochemical stability in saline, serum, in vitro binding with S. aureus and biodistribution in artificially infected with S. aureus MWR. The ^99mTc–RUN complex showed stability more than 90% up to 240 min in normal saline with a maximum stability value of 98.10 ± 0.18% at 30 min after reconstitution. At 37 °C the complex showed in vitro permanence in serum up to 16 h with 13.90% side products during incubation. The ^99mTc–RUN complex showed saturated in vitro binding with S. aureus at different intervals with a maximum uptake value of 71.50%. Infected to normal muscle, infected to inflamed and inflamed to normal muscles ratios were approximately 6.04, 4.31 and 1.40. Based on the stability of the complex in saline, serum, in vitro binding with S. aureus and biodistribution results, the ^99mTc–RUN complex is recommended for in vivo scintigraphic localization of the S. aureus in vivo infectious foci in human.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)