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  • 1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed to be University), Allahabad, Uttar Pradesh, India
  • | 2 Department of Tissue Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed to be University), Allahabad, Uttar Pradesh, India
  • | 3 Department of Biological Sciences, School of Basic Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed to be University), Allahabad, Uttar Pradesh, India
  • | 4 School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
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Abstract  

When a biological system is either accidentally or intentionally exposed to radiation, the energy absorbed triggers a number of successive events including damage to living tissues. Major radiation damage is due to the aqueous free radicals generated by the radiolysis of water. These free radicals act as molecular marauders and in turn damage DNA, mitochondrial membrane, lipid, cellular protein, resulting in cellular dysfunction and mortality. In view of the above mentioned facts an experiment was conducted to study the genotoxic effects of γ radiation and its dose effectiveness. The present experiment was conducted on samples of plasmid pBR322 DNA as the in vitro experimental model devoid of any DNA repair and replication machinery. The samples were exposed to different doses of gamma radiations from 1 to 200 Gy. Exposure of plasmid pBR322 DNA to γ radiation resulted in production of single strand breaks as a result of which, the supercoiled (SC) form was converted to relaxed form (RL). Exposure of radiation, even at very low dose of 1 Gy, exhibited a significant damage to DNA resulting in about 70% SC form and 30% RL form of DNA. At a dose of 10 Gy the SC form was reduced to about 37% and further 5% at a dose of 50 Gy with about 88.5 and 6.5% RL and linear (L) forms of DNA respectively. Thus, the disappearance of supercoiled form of plasmid pBR322 DNA was found to be directly related to radiation dose and exhibited a radiation dose dependent pattern.

Manuscript Submission: HERE

  • Impact Factor (2019): 1.137
  • Scimago Journal Rank (2019): 0.360
  • SJR Hirsch-Index (2019): 65
  • SJR Quartile Score (2019): Q3 Analytical Chemistry
  • SJR Quartile Score (2019): Q3 Health, Toxicology and Mutagenesis
  • SJR Quartile Score (2019): Q2 Nuclear Energy and Engineering
  • SJR Quartile Score (2019): Q3 Pollution
  • SJR Quartile Score (2019): Q3 Public Health, Environmental and Occupational Health
  • SJR Quartile Score (2019): Q3 Radiology, Nuclear Medicine and Imaging
  • SJR Quartile Score (2019): Q3 Spectroscopy
  • Impact Factor (2018): 1.186
  • Scimago Journal Rank (2018): 0.408
  • SJR Hirsch-Index (2018): 60
  • SJR Quartile Score (2018): Q2 Nuclear Energy and Engineering
  • SJR Quartile Score (2018): Q2 Pollution

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
4
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)

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