Journal of Radioanalytical and Nuclear Chemistry
Volume/Issue:
Volume 293: Issue 1
Direct labeling of doxorubicin with technetium-99m: its optimization, characterization and quality control
Authors: Faheem Rizvi 1 , Tanveer Bokhari 1 , S. Roohi 2 , and A. Mushtaq 2
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  • 1 Department of Chemistry, GC University, Faisalabad, Pakistan
  • 2 Isotope Production Division, PINSTECH, Nilore, Islamabad, Pakistan
DOI:
https://doi.org/10.1007/s10967-012-1662-9
Page Count:
303–307
Publication Date:
01 Jul 2012
Online Publication Date:
21 Feb 2012
Article Category:
Research Article
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Abstract  

Doxorubicin (DOX) is an anthracycline antineoplastic and one of the most potent and widely used drugs in clinical oncology. It is used in the treatment of a wide variety of cancers. The aim of this study was the direct labeling of DOX with 99mTc; its optimization, characterization and quality control of the radiolabeled DOX. Labeling efficiency was determined by paper chromatography. More than 92% labeling was obtained at pH 6–7, 10–12 μg stannous chloride and 200 μg of DOX. The stability of 99mTc–DOX was studied up to 5 h. All the experiments were performed at room temperature (25± 2 °C). The characterization of the labeling compound was performed by HPLC and electrophoresis. Electrophoresis indicates that labeled DOX has no charge and HPLC shows single specie of labeled compound.

Cover Journal of Radioanalytical and Nuclear Chemistry
Journal of Radioanalytical and Nuclear Chemistry
Print ISSN:
0236-5731
Online ISSN:
1588-2780
Keywords:
99mTc labeling; Doxorubicin; Characterization; Quality control; HPLC; Electrophoresis; Paper chromatography

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