Doxorubicin (DOX) is an anthracycline antineoplastic and one of the most potent and widely used drugs in clinical oncology.
It is used in the treatment of a wide variety of cancers. The aim of this study was the direct labeling of DOX with 99mTc; its optimization, characterization and quality control of the radiolabeled DOX. Labeling efficiency was determined by
paper chromatography. More than 92% labeling was obtained at pH 6–7, 10–12 μg stannous chloride and 200 μg of DOX. The stability
of 99mTc–DOX was studied up to 5 h. All the experiments were performed at room temperature (25± 2 °C). The characterization of the
labeling compound was performed by HPLC and electrophoresis. Electrophoresis indicates that labeled DOX has no charge and
HPLC shows single specie of labeled compound.