Tritium and 14C concentration in hot spring water in Korea were determined. Tritium in the most hot spring samples could not detected and concentration range was <0.5–1.31 TU. And 14C contents were ranged 1.7–78.6 pMC. From the 3H and 14C analysis, we found some hot springs are mixed with recent groundwater and hot spring water ages were estimated from 1,940 to 32,800 years. And also, 226Ra and 228Ra were determined simultaneously in hot spring water, and some other radionuclides were also analyzed. Content distribution of 222Rn was 50–3,760 pCi/L, 226Ra was <0.003–0.15 Bq/L and 228Ra was below the detection limit.