The use of15N-labelled amino acids in vivo and in vitro for transmination studies: Tryptophan transaminase in maize plants

M. Králová; M. Kutáček; P. Stránský

doi:10.1007/bf02516986

Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 30: Issue 2

The use of¹⁵N-labelled amino acids in vivo and in vitro for transmination studies: Tryptophan transaminase in maize plants

Authors:

M. Králová

M. Králová Czechoslovak Academy of Sciences Institute of Experimental Botany Prague (Czechoslovakia)

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M. Kutáček

M. Kutáček Czechoslovak Academy of Sciences Institute of Experimental Botany Prague (Czechoslovakia)

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, and

P. Stránský

P. Stránský Czechoslovak Academy of Sciences Institute of Experimental Botany Prague (Czechoslovakia)

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Pages:: 507–514

Online Publication Date:: 09 Jan 2007

Publication Date:: 01 Sep 1976

Article Category:: Research Article

DOI:: https://doi.org/10.1007/bf02516986

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Abstract

Besides conventional methods, transminase activity can also be followed by an isotopically labelled substrate, i.e. by an amino acid with a¹⁵N labelled amino group. The application of the labelled substrate enables their study in vitro as well as in vivo. This method was applied for following the tryptophan transaminase activity in germinating maize plants. The enzym preparations used for in vitro analysis were purified on a Sephadex G 100 column. For in vivo experiments the¹⁵ N substrate was introduced by vacuum infiltration into the plants leaves. The amino acids originated by transamination are chromatographically separated and after purifying by paper electrophoresis, the atom excess¹⁵N is determined. In maize tissue originated glutamic acid (from infiltrated α-ketoglutarate substrate), which in the course of further reactions was transformed into aspartic acid and asparagine. The results found so far by studying the low activity of tryptophan-transaminase in vivo entitle us to improve our method of transaminase activity determination by using¹⁵N labelled-substrate.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)