A rapid method for the determination of uranium at nanogram levels in geological and biological materials is described. The method is based on the separation of 23.5 min239U, produced on neutron activation, by solvent extraction with TBP from nitric acid medium in the presence of HF, which prevents interference from Th; a number of other possibly interfering elements were shown to be negligibly extracted. The method is selective and sensitive enough for the determination of nanogram levels of uranium in soils, ores and biological samples. Results for some standard reference materials were in good agreement with certified values.