Americium and curium are separated on a column of cation exchange resin (Aminex) using hydroxyisobutyric acid (α HIBA) as
eluent, at a temperature of 80°C. Americium and curium were detected on-line, using their α-emission; the separation was performed
in a shielded glove box whose setting-up is given. The time necessary for a separation was between 30 min and 1 hr. The purity
of separated fractions was assayed by mass spectrometry. An application to the determination of the isotopic composition of
americium and curium in fuels is described.