The possibility of a simultaneous determination of low and medium Z elements in hair in a single ion beam exposure was studied. Different ion beams, proton, deuteron,3He and18O, at velocities ranging from 2.7–6.9 MeV/amu were investigated. In this work,188O4+ beam was found to give the best experimental condition in terms of sensitivity and number of elements detected. The detection limits in a single hair ranged from 2.9·10−5 μg for Fe to 0.72 μg for H using this beam.