Tropolone-5-sulfonic acid was used to separate and collect trace elements as their complexes onto an anion-exchange resin
in photon-activation of biological materials. Retention behaviour of the anionic complexes of Cr, Mn, Fe, Co, Ni, Cu, Zn,
and Pb on Dowex 1-X8 resin was examined with respect to pH, amount of resin, concentration of the ligand, and coexisting salt
anions, using radioisotope tracers. Under optimum conditions, Cr, Mn, Co, Ni, Zn and Pb were collected from the irradiated
samples of NBS-SRM Orchard Leaves, NBS-SRM Spinach, green tea and black tea on the marcket. The analytical results agreed
with certified values and the reproducibility was good.