Authors:
M. Abdullah University of Riyadh Department of Pharmaceutics, College of Pharmacy Riyadh (Saudi Arabia)

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A. Shibl University of Riyadh Department of Pharmaceutics, College of Pharmacy Riyadh (Saudi Arabia)

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S. Said University of Riyadh Department of Pharmaceutics, College of Pharmacy Riyadh (Saudi Arabia)

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M. Abdulhafeez University of Riyadh Department of Pharmaceutics, College of Pharmacy Riyadh (Saudi Arabia)

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Abstract  

Protease, an enzyme obtained fromPseudomanas aeruginosa, was radioiodinated using chloramine T method of (125I) iodide oxidation. The labelled product was purified using Sephadex G-50. The radiochemical yield reached 26.36% with a product specific activity 6.25 μCi/mg. Blood level curves, following intravenous injection of the labelled protease into mice, showed a biphasic blood level with a rapid initial declining phase; the characteristic of two-compartment open model. The rate constants K21, K12, and K10 were 0.05044, 0.006236, and 0.008264 min−1 respectively indicating a sharp decline in the serum concentration of the toxin, with a t1/2 of 83.85 min. The organ uptake of radioactivity follows the sequence liver>kidneys>lungs>heart>spleen>thyroid. A correlation between uptake and the incidence of tissue-hemorrhage is discussed.

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)