Authors:
H. Lundqvist University of Uppsala Department of Physical Biology, The Gustaf Werner Institute Box 531 S-751 21 Uppsala (Sweden)

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B. Långström University of Uppsala Department of Organic Chemistry, Institute of Chemistry Box 531 S-751 21 Uppsala (Sweden)

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M. Malmqvist National Defence Research Institute Dept. 4 S-901 82 Umeå (Sweden)

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Abstract  

In syntheses of naturally occurring amino acids labelled with short-lived radionuclides, such as11C, the determination of the enantiomeric purity presents problems. The aminoacyl coupling of L-amino acids to tRNA followed by a separation with gel filtration of the tRNA-amino acid complex and free amino acid, was here shown to be an adequate method for determining the enantiomeric purity of [methyl-11C]-methionine even in the pmol range. This method has also been used for the determination of the specific radioactivity of the11C-labelled methionine. The method is probably valid for other naturally occurring amino acids and it might also be of interest for enantiomeric separations of L- and D-amino acids of high specific radioactivity.

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)