The extent of hydration of agar has been calculated from the self-diffusion studies of Br– and I– ions in agar gel medium. The measured obstruction effect caused by these macromolecules is related to the extent of hydration by Wang's equation. The value of hydration expressed in terms of grams of bound water per gram of anhydrous agar, in presence of NaI and NH4Br /0.1M/ electrolytes comes out to be about 5.2.