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  • 1 Bhabha Atomic Research Centre Radiopharmaceuticals Division 400 085 Bombay (India)
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We describe a radioimmunoassay procedure for human somatotropin using second antibody — polyethylene glycol (PEG) combination for the separation of antibody bound and free antigen. The assay is done by a single incubation of 18 hours or with one hour pre-incubation and 3 hours post-incubation at room temperature (25 °C) and uses 0.1 m of serum sample. The assay covers a range of 0–40 g/ and has a sensitivity of 0.6 g/ of somatotropin. The assay is validated by inter-assay and intra-assay variations, recovery and parallelism tests.