Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 100: Issue 1
A thermodynamic study of nickel ion interaction with bovine carbonic anhydrase II molecule
Authors:
G. BehbehaniChemistry Department, Imam Khomeini International University, Qazvin, Iran

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A. SabouryInstitute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

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E. YahaghiSchool of Chemistry, University of Tehran, Tehran, Iran

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Pages:
283–288
Online Publication Date:
10 Jun 2009
Publication Date:
01 Apr 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1007/s10973-009-0012-9
Keywords:
Bovine carbonic anhydrase; Nickel ion; Isothermal titration calorimetry
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Abstract  

A thermodynamic study on the interaction of bovine carbonic anhydrase II (CAII) with nickel ions was performed by using isothermal titration calorimetry (ITC) at 27 °C in Tris buffer solution at pH = 7.5. The enthalpies of Ni2+ + CAII interaction are reported and analysed in terms of the new solvation theory. It was indicated that there are three identical and non-cooperative sites for Ni2+. The binding of a nickle ion is exothermic with dissociation equilibrium constants of 81.306 and 99.126 μM at 27°C and 37°C, respectively. The binding of nickel ions can cause some changes in the stability of the enzyme at low and high Ni2+ concentrations.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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