By using the isothermal titration calorimetry (ITC) technique, thermodynamic parameters have been determined for reactions
of the Mg2+, Ca2+, Sr2+, and Ba2+ ions with the citrate anion. The measurements were run in the Cacodylate, Pipes and Mes buffer solutions of a pH of 6, at
298.15 K, as well as in the Tricine, Tapso, and Tris–HCl buffer solutions of a pH of 8. Further, based on the results of potentiometric
titration, the conditional stability constants were determined for the citrate complexes at both pH values. The effect of
the reaction environment and the metal ion identity on the interaction energy with the citrate ligand and the stability of
the resulting compounds have been discussed.