Authors:
Jianan Sun Department of Ophthalmology, Okayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama City 700-8558, Japan
Department of Ophthalmology, The Second Hospital Affiliated to Jilin University, Changchun, Jilin, People's Republic of China

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Toshihiko Matsuo Department of Ophthalmology, Okayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama City 700-8558, Japan

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Tetsuya Uchida Division of Chemistry and Biochemistry, Okayama University Graduate School of Natural Science and Technology, Okayama City, Japan

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Abstract

Trehalose, a non-reducing disaccharide, is known as an exceptional protector against desiccation and heat stress. Differential scanning calorimetry is a simple tool to determine thermodynamic parameters concerning the structural state of proteins. In this study, we measured denaturation temperature (or melting temperature) of dried lenses to know the effect of trehalose pretreatment. Isolated porcine lenses were incubated in wells without any solution or with saline, 100 mM trehalose, or 100 mM cyclic tetrasaccharide in saline at room temperature for 150 min. The solutions were removed, and all lens samples were dried at room temperature and in room humidity until the weight showed no change. The nucleus of each sample was taken out and placed on a measuring platform for differential scanning calorimetry. The denaturation temperature of the dried lenses significantly rose by about 10 °C with 100 mM trehalose pretreatment, compared to no pretreatment, pretreatment with saline or 100 mM cyclic tetrasaccharide (P < 0.05, analysis of variance, P < 0.05, Student–Newman–Keuls tests, n = 7). The denaturation temperature showed no difference among the dried lenses with no pretreatment or pretreatment by saline or cyclic tetrasaccharide. In conclusion, pretreatment with trehalose raises denaturation temperature of the dried porcine lens. Trehalose might stabilize the dried tissue structure to get a higher denaturation temperature.

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
1
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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