Authors:
J. Lerchner TU Bergakademie Freiberg, Institute of Physical Chemistry, Leipziger Str. 29, 09596, Freiberg, Germany

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D. Mueller-Hagen Polyphag GmbH, Viersen, Germany

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H. Roehr Polyphag GmbH, Viersen, Germany

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A. Wolf TU Bergakademie Freiberg, Institute of Physical Chemistry, Leipziger Str. 29, 09596, Freiberg, Germany

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F. Mertens TU Bergakademie Freiberg, Institute of Physical Chemistry, Leipziger Str. 29, 09596, Freiberg, Germany

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R. Mueller Polyphag GmbH, Viersen, Germany

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W. Witte Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany

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I. Klare Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany

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Abstract

Rapid detection of antibiotic resistances of clinical bacterial strains would allow an early selective antibiotic therapy and a faster intervention and implementation of infection control measurements. In clinical practice, however, conventional antibiotic susceptibility tests of bacteria often need 24 h until the results are obtained. The metabolic heat production of bacteria is an excellent possibility to record their physiological activities and could therefore be used for a rapid discrimination of bacterial strains which are resistant or non-resistant to antibiotics and also to lytic bacteriophages, respectively. Unfortunately, conventional calorimeters suffer from need of comparably large volumes of bacterial suspensions are characterised by slow operation and high costs which restrict their application in clinical laboratories. The present paper demonstrates that a new type of calorimeters developed on silicon-chip technology enables the detection of antibiotic resistances on a minute-timescale. For this reasons, a prototype chip calorimeter was used which sensitivity is 20 nW related to the heat production of about 104 bacteria. For a clear discrimination of antibiotic resistance about 105 bacteria are required. The antibiotic resistances and susceptibilities of different strains of Staphylococcus aureus to cefoxitin and the sensitivities of S. aureus DSM 18421 and E. coli DSM 498 to a mixture of two bacteriophages were studied. Comparing the heat productions of cultures incubated with antibiotics or bacteriophages to those without these antibacterial preparations enabled a clear discrimination of resistant and non-resistant strains already after totally 2 h.

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  • 2. Johnson, G, Millar, MR, Matthews, S, Skyrme, M, Marsh, P, Barringer, E, O'Hare, S, Wilks, M 2006 Evaluation of BaLite Rapid MRSA, a rapid culture based screening test for the detection of ciprofloxacin- and methicillin-resistant S. aureus (MRSA) from screening swabs. BMC Microbiol 6:83 .

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  • 3. Mardh, PA, Ripa, T, Andersson, KE, Wadsö, I 1976 Kinetics of the action of tetracyclines on Escherichia coli as studied by microcalorimetry. J Antimicrob Chemother 10:604609.

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  • 4. von Ah, U Wirz, D, Daniels, AU 2008 Rapid differentiation of methicillin-susceptible Staphylococcus aureus from methicillin-resistant S. aureus and MIC determinations by isothermal microcalorimetry. J Clin Microbiol 46:20832087 .

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  • 5. Lerchner, J, Wolf, A, Schneider, H-J, Mertens, F, Kessler, E, Baier, V, Funfak, A, Nietzsch, M, Kruegel, M 2008 Nano-calorimetry of small-sized biological samples. Thermochim Acta 477:4853 .

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  • 6. Lerchner, J, Wolf, A, Buchholz, F, Mertens, F, Neu, TR, Harms, H, Maskow, T 2008 Miniaturized calorimetry—a new method for real-time biofilm activity analysis. J Microbiol Methods 74:7481 .

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  • 7. Deutsches Institut für Normung DIN 58940. Medical microbiology—susceptibility testing of pathogens to antimicrobial agents. Part 8: microdilution. General method specific-requirements. In: DIN Deutsches Institut für Normung e.V., editor. DIN-Taschenbuch 222: Medizinische Mikrobiologie und Immunologie—Diagnostische Verfahren. Berlin: Beuth-Verlag; 2004. p. 34253.

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
1
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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