Authors:
,
,
,
,
, and
Yan-Ling Zhao
Yan-Ling Zhao
Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China
Search for other papers by Yan-Ling Zhao in
Current site
Google Scholar
PubMed
Search for other papers by Yan-Ling Zhao in
Current site
Google Scholar
PubMed
Jia-Bo Wang
Jia-Bo Wang
Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China
Search for other papers by Jia-Bo Wang in
Current site
Google Scholar
PubMed
Search for other papers by Jia-Bo Wang in
Current site
Google Scholar
PubMed
Ping Zhang
Ping Zhang
Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China
Search for other papers by Ping Zhang in
Current site
Google Scholar
PubMed
Search for other papers by Ping Zhang in
Current site
Google Scholar
PubMed
Li-Mei Shan
Li-Mei Shan
Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China
Search for other papers by Li-Mei Shan in
Current site
Google Scholar
PubMed
Search for other papers by Li-Mei Shan in
Current site
Google Scholar
PubMed
Rui-Sheng Li
Rui-Sheng Li
Animal Laboratory Center 302 Hospital of People's Liberation Army 100 The 4th Ring Road Beijing 100039 People's Republic of China
Search for other papers by Rui-Sheng Li in
Current site
Google Scholar
PubMed
Search for other papers by Rui-Sheng Li in
Current site
Google Scholar
PubMed
Xiao-He Xiao
Xiao-He Xiao
Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China
Search for other papers by Xiao-He Xiao in
Current site
Google Scholar
PubMed
Search for other papers by Xiao-He Xiao in
Current site
Google Scholar
PubMed
Abstract
Splenic lymphocytes play an important role in host acute or chronic diseases. The abnormality of these cells in the spleens of humans might lead to some riskful diseases for human. Hence, in this study, the effects of two ginsenosides Rg1 and Rb1 on splenic lymphocytes growth were studied by microcalorimetry. Some qualitative and quantitative information, such as the metabolic power-time curves, growth rate constant k, maximum heat-output power of the exponential phase Pmax, total heat output Qt of splenic lymphocytes were obtained to present the effects of Rg1 and Rb1 on these cells. The values of k, Pmax, and Qt from the thermogenic growth curves of splenic lymphocytes were found to increase in the presence of Rg1, while the change was adverse for Rb1, illustrating that Rg1 had promotion effect and Rb1 had inhibitory effect on splenic lymphocytes growth and these promotion or inhibitory effects were enhanced with increasing the concentration of the two compounds, respectively. The microcalorimetric results were confirmed by MTT assay for determining the MTT optical density (OD) value and [3H] Thymidine incorporation assay ([3H]-TdR) for determining the count per minute (cpm) value: Rg1 could increase the MTT OD value and the cpm value of [3H]-TdR incorporation into splenic lymphocytes, and these values were increased with increasing the concentration of this compound, while Rb1 had the adverse results. The structure–activity relationships showed that the glucopyranoside and hydroxyl groups at the dammarane-type mother nucleus skeleton might play a crucial role for the opposing effects of the two ginsenosides on splenic lymphocytes. Compared with the other two assay methods, the microcalorimetric method provided more useful and reliable information for quickly and objectively evaluating the effects of drugs or compounds on the living cells, which would be a highly promising analytical tool for the characterization of the biological process and the estimation of the drugs’ efficiency.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
SY Nah DH Kim H Rhim 2007 Ginsenosides: are any of them candidates for drugs acting on the central nervous system? CNS Drug Rev 13 381 404.
-
2.
SY Nah KS Bhatia J Lyles EH Ellinwood TH Lee 2009 Effects of ginseng saponin on acute cocaine-induced alterations in evoked dopamine release and uptake in rat brain nucleus accumbens Brain Res 1248 184 190 .
-
3.
CF Chen WF Chiou JT Zhang 2008 Comparison of the pharmacological effects of Panax ginseng and Panax quinquefolium Acta Pharmacol Sinica 29 1103 1108 .
-
4.
Shibata S , Tanaka O, Shoji J, Saito H. Chemistry and pharmacology of Panax. In: Wagner H, Hikino H, Farnsworth NR, editors. Economic and medicinal plant research, vol. 1. London, Orlando, SanDiego, New York, Toronto, Montreal, Sydney, Tokyo: Academic Press; 1985. p. 217–87.
-
5.
S Sengupta SA Toh LA Sellers JN Skepper P Koolwijk HW Leung HW Yeung RN Wong R Sasisekharan TP Fan 2004 Modulating angiogenesis: the yin and the yang in ginseng Circulation 110 1219 1225 .
-
6.
GS Panayi JS Lanchbury GH Kingsley 1992 The importance of the T-cell in initiating and maintaining the chronic synovitis of rheumatoid arthritis Arth Rheumatol 35 729 735 .
-
7.
KM Murphy 1998 T lymphocyte differentiation in the periphery Curr Opin Immunol 10 226 232 .
-
8.
WJ Kong JB Wang C Jin YL Zhao CM Dai XH Xiao ZL Li 2009 Effect of emodin on Candida albicans growth investigated by microcalorimetry combined with chemometric analysis Appl Microbiol Biotechnol 83 1183 1190 .
-
9.
WJ Kong C Jin XH Xiao YL Zhao ZL Li P Zhang W Liu XF Li 2010 Comparative study of effects of two bile acid derivatives on Staphylococcus aureus by multiple analytical methods J Hazard Mater 179 742 747 .
-
10.
I Wadsö 2009 Characterization of microbial activity in soil by use of isothermal microcalorimetry J Therm Anal Calorim 95 843 850 .
-
11.
AB Afzal MJ Akhtar LG Svensson 2010 Thermal studies of DBSA-doped polyaniline/PVC blends by isothermal microcalorimetry J Therm Anal Calorim 100 1017 1025 .
-
12.
H Zhao S Bennici J Shen A Auroux 2010 Calorimetric study of the acidic character of V2O5–TiO2/SO4 2− catalysts used in methanol oxidation to dimethoxymethane J Therm Anal Calorim 99 843 847 .
-
13.
Zhao YL , Yan D, Wang JB, Zhang P, Xiao XH. Anti-fungal effect of berberine on Candida albicans by microcalorimetry with correspondence analysis. J Therm Anal Calorim. doi:.
-
14.
Kong WJ , Li ZL, Xiao XH, Zhao YL, Zhang P. Activity of berberine on Shigella dysenteriae investigated by microcalorimetry and multivariate analysis. J Therm Anal Calorim. doi:.
-
15.
LN Yang LX Sun F Xu J Zhang JN Zhao ZB Zhao CG Song RH Wu R Ozao 2010 Inhibitory study of two cephalosporins on E. coli by microcalorimetry J Therm Anal Calorim 100 589 592 .
-
16.
X Li T Zhang XM Min P Liu 2010 Toxicity of aromatic compounds to Tetrahymena estimated by microcalorimetry and QSAR Aquat Toxicol 98 322 327 .
-
17.
WJ Kong YL Zhao XH Xiao ZL Li C Jin HB Li 2009 Investigation of the anti-fungal activity of coptisine on Candida albicans growth by microcalorimetry combined with principal component analysis J Appl Microbiol 107 1072 1080 .
-
18.
PJ Zhou HT Zhou P Yi Y Liu ZB Wu SS Qu YG Zhu 2001 Microcalorimetric studies on the thermogenesis of energy release of mitochondria isolated from rice Microchem J 69 103 109 .
-
19.
AM Tan B Xu SQ Huang SS Qu 1999 Thermochemical study on the growth metabolism of human promyelocytic leukemia HL-60 cells inhibited by water-soluble metalloporphyrins Thermochim Acta 333 99 102 .
-
20.
M Liu JT Zhang 1996 Studies on the mechanisms of immunoregulatory effects of ginsenoside Rg1 in aged rats Acta Pharm Sin 31 95 100.
-
21.
D Liu B Li Y Liu AS Attele JW Kyle CS Yuan 2001 Voltage-dependent inhibition of brain Na(+) channels by American ginseng Eur J Pharmacol 413 47 54 .
-
22.
Zhang GJ . Bioassay on traditional Chinese medicine. Beijing: China Chemical Industry Press; 2006.
-
23.
XH Xiao C Jin ZZ Zhao PG Xiao YY Wang 2007 Probe into innovation and development of pattern of quality control and evaluation for Chinese medicine China J Chin Mater Med 32 1377 1381.
-
24.
XH Xiao D Yan JB Wang C Jin 2009 Some opinions on bioassay for the quality control of Chinese medicines World Sci Tech/Mod Tradit Chin Med Mater Medica 11 504 508.
-
25.
O Braissant D Wirz B Göpfert AU Daniels 2010 Use of isothermal microcalorimetry to monitor microbial activities FEMS Microbiol Lett 33 1 8 .
To see the editorial board, please visit the website of Springer Nature.
Manuscript Submission: HERE
For subscription options, please visit the website of Springer Nature.
| Journal of Thermal Analysis and Calorimetry | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1969 |
| Volumes per Year |
1 |
| Issues per Year |
24 |
| Founder | Akadémiai Kiadó |
| Founder's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Publisher | Akadémiai Kiadó Springer Nature Switzerland AG |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 1388-6150 (Print) |
| ISSN | 1588-2926 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Dec 2024 | 57 | 1 | 0 |
| Jan 2025 | 96 | 0 | 0 |
| Feb 2025 | 92 | 0 | 0 |
| Mar 2025 | 88 | 1 | 0 |
| Apr 2025 | 23 | 0 | 0 |
| May 2025 | 2 | 0 | 0 |
| Jun 2025 | 0 | 0 | 0 |
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Powered by PubFactory