Authors:
Yan-Ling Zhao Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China

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Jia-Bo Wang Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China

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Ping Zhang Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China

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Li-Mei Shan Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China

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Rui-Sheng Li Animal Laboratory Center 302 Hospital of People's Liberation Army 100 The 4th Ring Road Beijing 100039 People's Republic of China

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Xiao-He Xiao Institute of Traditional Chinese Material Medica People's Liberation Army, 100 The 4th Ring Road Beijing 100039 People's Republic of China

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Abstract

Splenic lymphocytes play an important role in host acute or chronic diseases. The abnormality of these cells in the spleens of humans might lead to some riskful diseases for human. Hence, in this study, the effects of two ginsenosides Rg1 and Rb1 on splenic lymphocytes growth were studied by microcalorimetry. Some qualitative and quantitative information, such as the metabolic power-time curves, growth rate constant k, maximum heat-output power of the exponential phase Pmax, total heat output Qt of splenic lymphocytes were obtained to present the effects of Rg1 and Rb1 on these cells. The values of k, Pmax, and Qt from the thermogenic growth curves of splenic lymphocytes were found to increase in the presence of Rg1, while the change was adverse for Rb1, illustrating that Rg1 had promotion effect and Rb1 had inhibitory effect on splenic lymphocytes growth and these promotion or inhibitory effects were enhanced with increasing the concentration of the two compounds, respectively. The microcalorimetric results were confirmed by MTT assay for determining the MTT optical density (OD) value and [3H] Thymidine incorporation assay ([3H]-TdR) for determining the count per minute (cpm) value: Rg1 could increase the MTT OD value and the cpm value of [3H]-TdR incorporation into splenic lymphocytes, and these values were increased with increasing the concentration of this compound, while Rb1 had the adverse results. The structure–activity relationships showed that the glucopyranoside and hydroxyl groups at the dammarane-type mother nucleus skeleton might play a crucial role for the opposing effects of the two ginsenosides on splenic lymphocytes. Compared with the other two assay methods, the microcalorimetric method provided more useful and reliable information for quickly and objectively evaluating the effects of drugs or compounds on the living cells, which would be a highly promising analytical tool for the characterization of the biological process and the estimation of the drugs’ efficiency.

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
1
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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