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Yan-Ling Zhao Institute of Traditional Chinese Material Medica, People's Liberation Army, 100, The 4th Ring Road, Beijing 100039, People's Republic of China

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Jia-Bo Wang Institute of Traditional Chinese Material Medica, People's Liberation Army, 100, The 4th Ring Road, Beijing 100039, People's Republic of China

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Dan Yan Institute of Traditional Chinese Material Medica, People's Liberation Army, 100, The 4th Ring Road, Beijing 100039, People's Republic of China

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Li-Mei Shan Institute of Traditional Chinese Material Medica, People's Liberation Army, 100, The 4th Ring Road, Beijing 100039, People's Republic of China

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Ping Zhang Institute of Traditional Chinese Material Medica, People's Liberation Army, 100, The 4th Ring Road, Beijing 100039, People's Republic of China

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Rui-Sheng Li Animal Laboratory Center, 302 Hospital of People's Liberation Army, 100, The 4th Ring Road, Beijing 100039, People's Republic of China

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Xiao-He Xiao Institute of Traditional Chinese Material Medica, People's Liberation Army, 100, The 4th Ring Road, Beijing 100039, People's Republic of China

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Abstract

In this study, the activities of four ginsenosides Rc, Re, Rd, and Rf on splenic lymphocytes growth were studied by microcalorimetry. Some qualitative and quantitative information, such as the metabolic power–time curves, growth rate constant k, maximum heat-output power of the exponential phase Pmax and the corresponding appearance peak time tmax, total heat output Qt, and promotion rate Rp of splenic lymphocytes growth affected by the four ginsenosides were calculated. In accordance with thermo-kinetic model, the corresponding quantitative relationships of k, Pmax, tmax, Qt, Rp, and c were established. Also, the median effective concentration (EC50) was obtained by quantitative analysis. Based on both the quantitative quantity–activity relationships (QQAR) and EC50, the sequence of promotion activity was Rc > Re > Rd > Rf. The analysis of structure–activity relationships showed that the number, type, and position of sugar moieties on the gonane steroid nucleus had important influences on the promotion activity of Rc, Re, Rd, and Rf on splenic lymphocytes growth. Microcalorimetry can be used as a useful tool for determining the activity and studying the quantity–activity relationship of drugs on cell.

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
1
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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