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Yanling Zhao
Yanling Zhao
Institute of Traditional Chinese Material Medica, People’s Liberation Army, 100, the 4th Ring Road, Beijing 100039, People’s Republic of China
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Jiabo Wang
Jiabo Wang
Institute of Traditional Chinese Material Medica, People’s Liberation Army, 100, the 4th Ring Road, Beijing 100039, People’s Republic of China
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Limei Shan
Limei Shan
Institute of Traditional Chinese Material Medica, People’s Liberation Army, 100, the 4th Ring Road, Beijing 100039, People’s Republic of China
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Ruisheng Li
Ruisheng Li
Animal Laboratory Center, 302 Hospital of People’s Liberation Army, 100, the 4th Ring Road, Beijing 100039, People’s Republic of China
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Dan Yan
Dan Yan
Institute of Traditional Chinese Material Medica, People’s Liberation Army, 100, the 4th Ring Road, Beijing 100039, People’s Republic of China
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Xiaohe Xiao
Xiaohe Xiao
Institute of Traditional Chinese Material Medica, People’s Liberation Army, 100, the 4th Ring Road, Beijing 100039, People’s Republic of China
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Abstract
The power–time curves of mice splenic lymphocytes growth at 37 °C affected by ginsenoside Rh2 were determined by microcalorimetry using a 3114/3236 TAM air bioactivity monitor with ampoule mode. Then, the minimal inhibitory concentration (MIC) of Rh2 on splenic lymphocytes growth was determined by serial dilution method. From factor analysis (FA) on six quantitative thermokinetic parameters from the power–time curves, the activity of Rh2 on splenic lymphocytes could be quickly evaluated by analyzing the changes in the two main parameters: growth rate constant k, and maximum heat-output power, Pm. The results showed that Rh2 had strong inhibitory activity on splenic lymphocytes growth, and this inhibitory activity was strengthened with increasing concentration of Rh2 in the concentration range of 1.0–32.0 μg mL−1. This strong inhibitory also could be confirmed from the MIC of 50.0 μg mL−1 of Rh2 on splenic lymphocytes growth in RPMI-1640 culture medium. This study illustrated that microcalorimetry could not only offer a useful method for evaluating the activity of drugs, but also serve as a quantitative, sensitive, and simple analytic tool for the evaluation of drugs on cell growth.
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| Journal of Thermal Analysis and Calorimetry | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1969 |
| Volumes per Year |
1 |
| Issues per Year |
24 |
| Founder | Akadémiai Kiadó |
| Founder's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Publisher | Akadémiai Kiadó Springer Nature Switzerland AG |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 1388-6150 (Print) |
| ISSN | 1588-2926 (Online) |
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