Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 107: Issue 1
Exploring antibiotic resistant mechanism by microcalorimetry
Determination of thermokinetic parameters of metallo-β-lactamase L1 catalyzing penicillin G hydrolysis
Authors:
Hui-Zhou Gao Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Qi Yang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Xiao-Yan Yan Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Zhu-Jun Wang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Ji-Li Feng Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Xia Yang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Sheng-Li Gao Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Lei Feng Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Xu Cheng Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Chao Jia Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Ke-Wu Yang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, People’s Republic of China

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Pages:
321–324
Online Publication Date:
17 Feb 2011
Publication Date:
01 Jan 2012
Article Category:
Brief Report
DOI:
https://doi.org/10.1007/s10973-011-1362-7
Keywords:
Microcalorimetry; Metallo-β-lactamases; L1; Antibiotic hydrolysis; Thermokinetic parameters
Restricted access

Abstract

In an effort to probe the reaction of antibiotic hydrolysis catalyzed by B3 metallo-β-lactamase (MβL), the thermodynamic parameters of penicillin G hydrolysis catalyzed by MβL L1 from Stenotrophomonas maltophilia were determined by microcalorimetric method. The values of activation free energy ΔG≠θ are 88.26, 89.44, 90.49, and 91.57 kJ mol−1 at 293.15, 298.15, 303.15, and 308.15 K, respectively, activation enthalpy ΔH≠θ is 24.02 kJ mol−1, activation entropy ΔS≠θ is −219.2511 J mol−1 K−1, apparent activation energy E is 26.5183 kJ mol−1, and the reaction order is 1.0. The thermodynamic parameters reveal that the penicillin G hydrolysis catalyzed by MβL L1 is an exothermic and spontaneous reaction.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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