View More View Less
  • 1 Department of Biotechnological Processes, School of Chemical Engineering, State University of Campinas (UNICAMP), Av. Albert Einstein, 500 São Paulo, Campinas 13083-970, Brazil
  • 2 Faculty of Health Sciences, Fernando Pessoa University (FCS-UFP), Rua Carlos da Maia, 296, 4200-150, Porto, Portugal
  • 3 Faculty of Animal Science and Food Engineering, University of São Paulo (USP), São Paulo, Brazil
  • 4 Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-UTAD/IBB), P.O. Box 1013, 5001-801, Vila Real, Portugal
Restricted access

Abstract

This study focuses on the physicochemical characterization of lipid materials useful for the production of the so-called solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC). The chosen lipids were Dynasan®114 (glyceril trimyristate) and Dynasan®118 (glyceril tristearate) as solid lipids (SL), melting temperature above 80 °C, and Miglyol®812 (caprylic/capric triglyceride) and Miglyol®840 (propylene glycol dicaprylate/dicaprate) as liquid lipids (LL), crystallizing below −15 °C. Raw lipids (pure or SL:LL mixtures) were analyzed by differential scanning calorimetry (DSC), wide angle X-ray diffraction (WAXD), and Polarized Light Microscopy (PLM), before and after tempering at 80 °C for 1 h. The selected SL:LL combination was 70% (Dynasan®114 and 118) and 30% (Miglyol®812 and 840) for the production of SLN and NLC by high-pressure homogenization (HPH), respectively. Particles with a mean size of 200 nm (polydispersity index <0.329) and zeta potential of −15 mV were obtained, and their long-term stability was confirmed for 3 months of storage at 7 °C.

  • 1. Sahoo SK , Parveen S, Panda JJ. The present and future of nanotechnology in human health care. Nanomedicine. 2007. .

  • 2. Aji Alex MR , Chacko AJ, Jose S, Souto EB. Lopinavir loaded solid lipid nanoparticles (SLN) for intestinal lymphatic targeting. Eur J Pharm Sci. 2011. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 3. Gonzalez-Mira E , Egea MA, Garcia ML, Souto EB. Design and ocular tolerance of flurbiprofen loaded ultrasound-engineered NLC. Colloids Surf B Biointerfaces. 2010. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4. Heney M , Alipour M, Vergidis D, Omri A, Mugabe C, Th’ng J, Suntres Z. Effectiveness of liposomal paclitaxel against MCF-7 breast cancer cells. Can J Physiol Pharmacol. 2010. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5. Wang J , Liu W, Tu Q, Song N, Zhang Y, Nie N. Folate-decorated hybrid polymeric nanoparticles for chemically and physically combined paclitaxel loading and targeted delivery. Biomacromolecules. 2011. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6. Barata TS , Shaunak S, Teo I, Zloh M, Brocchini S. Structural studies of biologically active glycosylated polyamidoamine (PAMAM) dendrimers. J Mol Model. 2010. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7. Rawat MK , Jain A, Singh S. In vivo and cytotoxicity evaluation of repaglinide-loaded binary solid lipid nanoparticles after oral administration to rats. J Pharm Sci. 2011. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8. Souto EB , Muller RH. Lipid nanoparticles: effect on bioavailability and pharmacokinetic changes. Handb Exp Pharmacol. 2010. .

  • 9. Muller, RH, Mader, K, Gohla, S. Solid lipid nanoparticles (SLN) for controlled drug delivery—a review of the state of the art. Eur J Pharm Biopharm. 2000;50: 1 161177. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10. De Oliveira GG , Ferraz HG, Severino P, Souto EB. Analysis of phase transition and dehydration processes of nevirapine. J Therm Anal Calorim. 2011. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 11. Yoshid MI , Oliveira MA, Gomes ECL, Mussel WN, Castro WV, Soares CDV. Thermal characterization of lovastatin in pharmaceutical formulations. J Therm Anal Calorim. 2011. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12. Jores K , Mehnert W, Drechsler M, Bunjes H, Johann C, Mader K. Investigations on the structure of solid lipid nanoparticles (SLN) and oil-loaded solid lipid nanoparticles by photon correlation spectroscopy, field-flow fractionation and transmission electron microscopy. J Control Release. 2004. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13. Bondi ML , Craparo EF, Giammona G, Drago F. Brain-targeted solid lipid nanoparticles containing riluzole: preparation, characterization and biodistribution. Nanomedicine (Lond). 2009. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14. Souto EB , Muller RH. Investigation of the factors influencing the incorporation of clotrimazole in SLN and NLC prepared by hot high-pressure homogenization. J Microencapsul. 2006. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15. Ali H , Shirode AB, Sylvester PW, Nazzal S. Preparation, characterization, and anticancer effects of simvastatin-tocotrienol lipid nanoparticles. Int J Pharm. 2010. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16. Wissing SA , Kayser O, Muller RH. Solid lipid nanoparticles for parenteral drug delivery. Adv Drug Deliv Rev. 2004. .

  • 17. Muchow M , Maincent P, Muller RH. Lipid nanoparticles with a solid matrix (SLN, NLC, LDC) for oral drug delivery. Drug Dev Ind Pharm. 2008. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 18. Mehnert, W, Mader, K. Solid lipid nanoparticles: production, characterization and applications. Adv Drug Deliv Rev. 2001;47:165196. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19. Lippacher, A, Muller, RH, Mader, K. Preparation of semisolid drug carriers for topical application based on solid lipid nanoparticles. Int J Pharm. 2001;214:912. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20. Muller, RH, Radtke, M, Wissing, SA. Nanostructured lipid matrices for improved microencapsulation of drugs. Int J Pharm. 2002;242:121128. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21. Muller, RH, Radtke, M, Wissing, SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Deliv Rev. 2002;54:S131S155. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 22. Souto EB , Mehnert W, Muller RH. Polymorphic behaviour of Compritol888 ATO as bulk lipid and as SLN and NLC. J Microencapsul. 2006. .

  • 23. Potta SG , Minemi S, Nukala RK, Peinado C, Lamprou DA, Urquhart A, Douroumis D. Preparation and characterization of ibuprofen solid lipid nanoparticles with enhanced solubility. J Microencapsul. 2011. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 24. Luykx DM , Peters RJ, van Ruth SM, Bouwmeester H. A review of analytical methods for the identification and characterization of nano delivery systems in food. J Agric Food Chem. 2008. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 25. Rica RA , Jiménez ML, Delgado AV. Dynamic mobility of rodlike goethite particles. Langmuir. 2009. .

  • 26. Jenning, V, Thunemann, AF, Gohla, SH. Characterisation of a novel solid lipid nanoparticle carrier system based on binary mixtures of liquid and solid lipids. Int J Pharm. 2000;199:167177. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 27. Mezzena M , Scalia S, Young PM, Traini D. Solid lipid budesonide microparticles for controlled release inhalation therapy. AAPS J. 2009. .

  • 28. Kim JK , Park JS, Kim CK. Development of a binary lipid nanoparticles formulation of itraconazole for parenteral administration and controlled release. Int J Pharm. 2010. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 29. Attama AA , Muller-Goymann CC. Investigation of surface-modified solid lipid nanocontainers formulated with a heterolipid-templated homolipid. Int J Pharm. 2007. .

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 30. Bayindir ZS , Yuksel N. Characterization of niosomes prepared with various nonionic surfactants for paclitaxel oral delivery. J Pharm Sci. 2010. .

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Sep 2020 5 0 1
Oct 2020 11 0 0
Nov 2020 6 1 0
Dec 2020 2 0 0
Jan 2021 5 0 0
Feb 2021 6 0 0
Mar 2021 3 0 0