This study focuses on the physicochemical characterization of lipid materials useful for the production of the so-called solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC). The chosen lipids were Dynasan®114 (glyceril trimyristate) and Dynasan®118 (glyceril tristearate) as solid lipids (SL), melting temperature above 80 °C, and Miglyol®812 (caprylic/capric triglyceride) and Miglyol®840 (propylene glycol dicaprylate/dicaprate) as liquid lipids (LL), crystallizing below −15 °C. Raw lipids (pure or SL:LL mixtures) were analyzed by differential scanning calorimetry (DSC), wide angle X-ray diffraction (WAXD), and Polarized Light Microscopy (PLM), before and after tempering at 80 °C for 1 h. The selected SL:LL combination was 70% (Dynasan®114 and 118) and 30% (Miglyol®812 and 840) for the production of SLN and NLC by high-pressure homogenization (HPH), respectively. Particles with a mean size of 200 nm (polydispersity index <0.329) and zeta potential of −15 mV were obtained, and their long-term stability was confirmed for 3 months of storage at 7 °C.
1. SahooSK, ParveenS, PandaJJ. The present and future of nanotechnology in human health care. Nanomedicine. 2007. .
Muller, RH, Mader, K, Gohla, S. Solid lipid nanoparticles (SLN) for controlled drug delivery—a review of the state of the art. Eur J Pharm Biopharm. 2000;50: 1161–177. 10.1016/S0939-6411(00)00087-4.)| false
12. JoresK, MehnertW, DrechslerM, BunjesH, JohannC, MaderK. Investigations on the structure of solid lipid nanoparticles (SLN) and oil-loaded solid lipid nanoparticles by photon correlation spectroscopy, field-flow fractionation and transmission electron microscopy. J Control Release. 2004. .
JoresK, MehnertW, DrechslerM, BunjesH, JohannC, MaderK. Investigations on the structure of solid lipid nanoparticles (SLN) and oil-loaded solid lipid nanoparticles by photon correlation spectroscopy, field-flow fractionation and transmission electron microscopy. J Control Release. 2004. 10.1016/j.jconrel.2003.11.012.)| false
SoutoEB, MullerRH. Investigation of the factors influencing the incorporation of clotrimazole in SLN and NLC prepared by hot high-pressure homogenization. J Microencapsul. 2006. 10.1080/02652040500435295.)| false
Lippacher, A, Muller, RH, Mader, K. Preparation of semisolid drug carriers for topical application based on solid lipid nanoparticles. Int J Pharm. 2001;214:9–12. 10.1016/S0378-5173(00)00623-2.)| false
Muller, RH, Radtke, M, Wissing, SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Deliv Rev. 2002;54:S131–S155. 10.1016/S0169-409X(02)00118-7.)| false
LuykxDM, PetersRJ, van RuthSM, BouwmeesterH. A review of analytical methods for the identification and characterization of nano delivery systems in food. J Agric Food Chem. 2008. 10.1021/jf8013926.)| false
Jenning, V, Thunemann, AF, Gohla, SH. Characterisation of a novel solid lipid nanoparticle carrier system based on binary mixtures of liquid and solid lipids. Int J Pharm. 2000;199:167–177. 10.1016/S0378-5173(00)00378-1.)| false
KimJK, ParkJS, KimCK. Development of a binary lipid nanoparticles formulation of itraconazole for parenteral administration and controlled release. Int J Pharm. 2010. 10.1016/j.ijpharm.2009.09.008.)| false