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  • 1 Department of Physics and Astronomy, Center for Nanoscopic Physics, Tufts University, Medford, MA, 02155, USA
  • | 2 Department of Chemistry, Tufts University, Medford, MA, 02155, USA
  • | 3 Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA
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Abstract

We used advanced thermal analysis methods to characterize a new family of A-B di-block copolymers based on the amino acid sequences of Nephila clavipes major ampulate dragline spider silk. Using temperature modulated differential scanning calorimetry with a thermal cycling method and thermogravimetry, we captured the effect of bound water acting as a plasticizer for spider silk-like biopolymer films which had been cast from water solution and then dried. A low glass transition because of bound water removal was observed in the first heating cycle, after which, a shift of glass transition was observed in A-block film due to crystallization and annealing, and in BA film due to annealing. No shift of glass transition after bound water removal was observed in B-block film. The reversing heat capacities, Cp, for temperatures below and above the glass transition were measured and compared to the calculated values. The solid state heat capacity was modeled below Tg, based on the vibrational motions of the constituent poly(amino acid)s, heat capacities of which are known from the ATHAS Data Bank. Excellent agreement was found between the measured and calculated values of the heat capacity, showing that this model can serve as a standard method to predict the solid state Cp for other biologically inspired block-copolymers. We also calculated the liquid state heat capacities of the 100% amorphous biopolymer at Tg, and this predicted value can be use to determined the crystallinity of protein-based materials.

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  • SJR Hirsch-Index (2019): 87
  • SJR Quartile Score (2019): Q3 Condensed Matter Physics
  • SJR Quartile Score (2019): Q3 Physical and Theoretical Chemistry
  • Impact Factor (2018): 2.471
  • Scimago Journal Rank (2018): 0.634
  • SJR Hirsch-Index (2018): 78
  • SJR Quartile Score (2018): Q2 Condensed Matter Physics
  • SJR Quartile Score (2018): Q2 Physical and Theoretical Chemistry

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
4
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)