Authors:
,
,
,
,
,
,
,
,
, and
Xia Yang
Xia Yang
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Xia Yang in
Current site
Google Scholar
PubMed
Search for other papers by Xia Yang in
Current site
Google Scholar
PubMed
Lei Feng
Lei Feng
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Lei Feng in
Current site
Google Scholar
PubMed
Search for other papers by Lei Feng in
Current site
Google Scholar
PubMed
Kang-Zhen Xu
Kang-Zhen Xu
School of Chemical Engineering, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Kang-Zhen Xu in
Current site
Google Scholar
PubMed
Search for other papers by Kang-Zhen Xu in
Current site
Google Scholar
PubMed
Hui-Zhou Gao
Hui-Zhou Gao
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Hui-Zhou Gao in
Current site
Google Scholar
PubMed
Search for other papers by Hui-Zhou Gao in
Current site
Google Scholar
PubMed
Chao Jia
Chao Jia
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Chao Jia in
Current site
Google Scholar
PubMed
Search for other papers by Chao Jia in
Current site
Google Scholar
PubMed
Cheng-Cheng Liu
Cheng-Cheng Liu
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Cheng-Cheng Liu in
Current site
Google Scholar
PubMed
Search for other papers by Cheng-Cheng Liu in
Current site
Google Scholar
PubMed
Jian-Min Xiao
Jian-Min Xiao
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Jian-Min Xiao in
Current site
Google Scholar
PubMed
Search for other papers by Jian-Min Xiao in
Current site
Google Scholar
PubMed
Le Zhai
Le Zhai
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Le Zhai in
Current site
Google Scholar
PubMed
Search for other papers by Le Zhai in
Current site
Google Scholar
PubMed
Li-Sheng Zhou
Li-Sheng Zhou
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Li-Sheng Zhou in
Current site
Google Scholar
PubMed
Search for other papers by Li-Sheng Zhou in
Current site
Google Scholar
PubMed
Ke-Wu Yang
Ke-Wu Yang
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China
Search for other papers by Ke-Wu Yang in
Current site
Google Scholar
PubMed
Search for other papers by Ke-Wu Yang in
Current site
Google Scholar
PubMed
Abstract
In an effort to understand the reaction of antibiotic hydrolysis with B2 metallo-β-lactamases (MβLs), the thermodynamic parameters of imipenem hydrolysis catalyzed by metallo-β-lactamase ImiS from Aeromonas veronii bv. sobria were determined by microcalorimetric method. The values of activation free energy 
are 86.400 ± 0.043, 87.543 ± 0.034, 88.772 ± 0.024, and 89.845 ± 0.035 kJ mol−1 at 293.15, 298.15, 303.15, and 308.15 K, respectively, activation enthalpy 
is 18.586 ± 0.009 kJ mol−1, activation entropy 
is −231.34 ± 0.12 J mol−1 K−1, apparent activation energy E is 21.084 kJ mol−1, and the reaction order is 1.5. The thermodynamic parameters reveal that the imipenem hydrolysis catalyzed by metallo-β-lactammase ImiS is an exothermic and spontaneous reaction.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Walsh, CT, Wright, GD. 2005. Introduction: antibiotic resistance. Chem Rev. 105:391–393 .
-
2.
Neu, HC. 1992. The crisis in antibiotic resistance. Science. 257:1064–1073 .
-
3.
Rasmussen, BA, Bush, K. 1997. Carbapenem-hydrolyzing β-lactamases. Antimicrob Agents Chemother. 41:223–232.
-
4.
Ronald, WR, Kenneth, JW, Lisa, DM, Alan, WD, Richard, H, Robert, TG, James, PS, Jordan, H. 1989. Studies on the structures of imipenem, dehydropeptidase I hydrolyzed imipenem, and related analogues. J Org Chem. 54:653–660 .
-
5.
Galleni, M, Lamotte-Brasseur, J, Rossolini, GM, Spencer, J, Dideberg, O, Frere, JM The Metallo-β-Lactamase Working Group 2001. Standard numbering scheme for class B β-lactamses. Antimicrob Agents Chemother. 45:660–663 .
-
6.
Frere, JM, Dubus, A, Galleni, M, Matagne, A, Amicosante, G. 1999. Mechanistic diversity of β-lactamases. Biochem Soc Trans. 27:58–63.
-
7.
Fabiane, SM, Sohi, MK, Wan, T, Payne, DJ, Bateson, JH, Mitchell, T, Sutton, BJ. 1998. Crystal structure of the zinc-dependent β-lactamase from Bacillus cereus at 1.9 Å resolution: binuclear active site with features of a mononuclear enzyme. Biochemistry. 37:12404–12411 .
-
8.
Hernandez Valladares, M, Felici, A, Weber, G, Adolph, HW, Zeppezauer, M, Rossolini, GM, Amicosante, G, Frere, JM, Galleni, M. 1997. Zn(II) dependence of the Aeromonas hydrophila AE036 metallo-beta-lactamase activity and stability. Biochemistry. 36:11534–11541 .
-
9.
LeBlond, C, Wang, J, Larsen, RD, Orella, CJ, Forman, AL, Landau, RN et al. 1996. Reaction calorimetry as an in situ kinetic tool for characterizing complex reactions. Thermochim Acta. 289 2 189–207 .
-
10.
Zhao YL , Wang JB, Zhang P, Shan LM, Li RS, Xiao XH. Microcalorimetric study of the opposing effects of ginsenosides Rg1 and Rb1 on the growth of micesplenic lymphocytes. J Therm Anal Calorim. 2011. doi: .
-
11.
Garcia-Cuello, V, Moreno-Pirajá, JC, Giraldo-Gutiérrez, L, Sapag, K, Zgrablich, G. 2009. A new microcalorimeter of adsorption for the determination of differential enthalpies. Microporous Mesoporous Mater. 120:239–245 .
-
12.
Yang, L, Sun, L, Xu, F, Zhang, J, Zhao, J, Zhao, Z et al. 2010. Inhibitory study of two cephalosporins on E. coli by microcalorimetry. J Therm Anal Calorim. 100 2 589–592 .
-
13.
Gao HZ , Yang Q, Yan XY, Wang ZJ, Feng JL, Yang X, et al. Exploring antibiotic resistant mechanism by microcalorimetry: determination of thermokinetic parameters of metallo-β-lactamase L1 catalyzing penicillin G hydrolysis. J Therm Anal Calorim. 2011. doi: .
-
14.
Crawford, PA, Sharma, N, Chandrasekar, S, Sigdel, T, Walsh, TR, Spencer, J et al. 2004. Over-expression, purification, and characterization of metallo-β-lactamase ImiS from Aeromonas Veronii bV Sobria. Protein Expr Purif. 36:272–279 .
-
15.
Bradford, MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254 .
-
16.
Marthada, VK. 1980. The enthalpy of solution of SRM 1655(KCl) in H2O. J Res Nat Bur Stand. 85 6 467–471 .
-
17.
Ditmars, DA, Ishihara, S, Chang, SS. 1982. Enthalpy and heat-capacity standard reference material: synthetic sapphire (α-Al2O3) from 10 to 2250 K. J Res N at l Bur Stand. 87:159–163 .
-
18.
Gao, S, Chen, S, Hu, R, Li, H, Shi, Q. 2002. Derivation and application of thermodynamic equations. Chin J Inorg Chem. 18 4 362–366.
-
19.
Spencer, J, Clarke, AR, Walsh, TR. 2001. Novel mechanism of hydrolysis of therapeutic beta-lactams by Stenotrophomonas maltophilia L1 metallo-β-lactamase. J Biol Chem. 276:33638–33644 .
-
20.
Feng, JL, Yang, X, Yan, XY, Gao, HZ, Wu, D, Yang, KW. 2011. Expression, purification of ImiS and kinetic studies on hydrolysis of three types of β-lactam antibiotics catalyzed by ImiS. Chin J Antibiotic. 36 3 197–200.
-
21.
Yan, XY, Gao, HZ, Feng, JL, Yang, X, Cheng, X, Jia, C, Yang, KW. 2011. Wild-type and Co(II) substituted metallo-β-lactamase L1: spectroscopic characterization and kinetic studies of catalyzing antibiotic hydrolysis. Chin J Antibiotic. 36 5 388–393.
To see the editorial board, please visit the website of Springer Nature.
Manuscript Submission: HERE
For subscription options, please visit the website of Springer Nature.
| Journal of Thermal Analysis and Calorimetry | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1969 |
| Volumes per Year |
1 |
| Issues per Year |
24 |
| Founder | Akadémiai Kiadó |
| Founder's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Publisher | Akadémiai Kiadó Springer Nature Switzerland AG |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 1388-6150 (Print) |
| ISSN | 1588-2926 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Nov 2024 | 74 | 0 | 0 |
| Dec 2024 | 19 | 0 | 0 |
| Jan 2025 | 37 | 0 | 0 |
| Feb 2025 | 54 | 0 | 0 |
| Mar 2025 | 52 | 0 | 0 |
| Apr 2025 | 22 | 0 | 0 |
| May 2025 | 0 | 0 | 0 |
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Powered by PubFactory