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Xia Yang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Lei Feng Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Kang-Zhen Xu School of Chemical Engineering, Northwest University, Xi'an 710069, People's Republic of China

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Hui-Zhou Gao Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Chao Jia Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Cheng-Cheng Liu Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Jian-Min Xiao Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Le Zhai Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Li-Sheng Zhou Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Ke-Wu Yang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, People's Republic of China

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Abstract

In an effort to understand the reaction of antibiotic hydrolysis with B2 metallo-β-lactamases (MβLs), the thermodynamic parameters of imipenem hydrolysis catalyzed by metallo-β-lactamase ImiS from Aeromonas veronii bv. sobria were determined by microcalorimetric method. The values of activation free energy are 86.400 ± 0.043, 87.543 ± 0.034, 88.772 ± 0.024, and 89.845 ± 0.035 kJ mol−1 at 293.15, 298.15, 303.15, and 308.15 K, respectively, activation enthalpy is 18.586 ± 0.009 kJ mol−1, activation entropy is −231.34 ± 0.12 J mol−1 K−1, apparent activation energy E is 21.084 kJ mol−1, and the reaction order is 1.5. The thermodynamic parameters reveal that the imipenem hydrolysis catalyzed by metallo-β-lactammase ImiS is an exothermic and spontaneous reaction.

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
1
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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