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  • 1 Faculte de Pharmacie Laboratoire de Physique Pharmaceutique 27, BD Jean Moulin 13385 Marseille France
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Direct microcalorimetric measurements allow determination of both the δH and association constant of biological complexes ifKa value does not exceed 106M−1. For higherKa values, δH can obviously be determined; this paper describes an original microcalorimetric method that permits determination of such high association constants. This method is based on the analysis of the competitive effect between two ligands having the same binding site in their receptor. As an example, the affinity constant for thymidylate synthase of a novel antifolate, CB 3717. was found to be 1.4 · 107M−1 using methotrexate polyglutamate MTX-G2 (Ka=2.3·105M−1) as competitor.

Manuscript Submission: HERE

  • Impact Factor (2019): 2.731
  • Scimago Journal Rank (2019): 0.415
  • SJR Hirsch-Index (2019): 87
  • SJR Quartile Score (2019): Q3 Condensed Matter Physics
  • SJR Quartile Score (2019): Q3 Physical and Theoretical Chemistry
  • Impact Factor (2018): 2.471
  • Scimago Journal Rank (2018): 0.634
  • SJR Hirsch-Index (2018): 78
  • SJR Quartile Score (2018): Q2 Condensed Matter Physics
  • SJR Quartile Score (2018): Q2 Physical and Theoretical Chemistry

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
per Year
per Year
Founder Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)