Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 38: Issue 9
Flow-microcalorimetric determination of enzymatic activities of the Trichoderma viridae-cellulase complex
Authors:
M. Beran Food Industry Research Institute Radiová 7 102 31 Praha 10 Czech and Slovak F.R.

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and
V. Paulicek Food Industry Research Institute Radiová 7 102 31 Praha 10 Czech and Slovak F.R.

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Pages:
1979–1988
Online Publication Date:
02 Aug 2005
Publication Date:
01 Sep 1992
Article Category:
Research Article
DOI:
https://doi.org/10.1007/bf01979607
Keywords:
cellulase complex; enzymatic activities; flow-microcalorimetryl; kinetics
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A method for measuring enzymatic activities of the Trichoderma viridae cellulase complex is described. The LKB 2277 Thermal activity monitor and a flow-mix mode were used. Enzymatic activities of samples of a crude cellulose complex have been determined using three substrates: cellobiose, carboxymethyl cellulose (CMC) and xylan. Some kinetic constants for cellobiase activity have been evaluated from the obtained results. A process of the end-product inhibition of cellobiase resp. carboxymethyl cellulase activity by glucose has been observed too.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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