Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 44: Issue 6
Methotrexate interaction with a lipid membrane model of DPPC
Authors:
G. Puglisi Istituto di Chimica Farmaceutica e Tossicologica, Università di Catania, V. le A. Doria 6, 95125 Catania, Italy

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M. Fresta Institut für Polymere, Eidgenössiche Technische Hochschule, Universitätstrasse 6, CH-8092 Zürich, Switzerland

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C. Ventura Istituto di Chimica Farmaceutica e Tossicologica, Università di Catania, V. le A. Doria 6, 95125 Catania, Italy

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G. Mazzone Istituto di Chimica Farmaceutica e Tossicologica, Università di Catania, V. le A. Doria 6, 95125 Catania, Italy

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M. Vandelli Departimento di Science Farmaceutiche, Università of Modena, Via G. Campi 183, 41100 Modena, Italy

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Pages:
1287–1299
Online Publication Date:
09 Jan 2007
Publication Date:
01 Jun 1995
Article Category:
Research Article
DOI:
https://doi.org/10.1007/bf02549218
Keywords:
DSC; interaction; lipid model membrane; methotrexate
Restricted access

Abstract  

Dipalmitoylphosphatidylcholine (DPPC) liposomes were employed as membrane models for the investigation of the interaction occurring between methotrexate (MTX) and bilayer lipid matrix. Liposomes were obtained by hydrating a lipid film with 50 mM Tris buffer (pH 7.4). The differential scanning calorimetry (DSC) evaluation of the thermotropic parameters associated with the phase transitions of DPPC liposomes gave useful information about the kind of drug-membrane interaction. The results showed an electrostatic interaction taking place with the negatively charged molecules of MTX and the phosphorylcholine head groups, constituting the outer part of DPPC bilayers. No interaction with the hydrophobic phospholipid bilayer domains was detected, revealing a poor capability of MTX to cross through lipid membranes to reach the interior compartment of a lipid bounded structure. These findings correlate well within vitro biological experiments on MTX cell susceptibility.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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