Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 47: Issue 5
Storage stability of the solution formulation of sCD4 determined by DSC in comparison with two functional assays
Authors:
C. G. Brouillette

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S. W. Tendian Southern Research Institute 2000 9th Ave. S. 35205 Birmingham AL

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B. C. Heard Southern Research Institute 2000 9th Ave. S. 35205 Birmingham AL

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D. Dunleavy

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A. L. Shorter

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D. G. Myszka

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I. M. Chaiken

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Pages:
1597–1609
Online Publication Date:
05 Aug 2005
Publication Date:
01 Nov 1996
Article Category:
Research Article
DOI:
https://doi.org/10.1007/bf01992848
Keywords:
affinity chromatography; biopharmaceutics; conformational epitope; differential scanning calorimetry; ELISA; gp120; multidomain protein; OKT4a; protein stability; sCD4
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Differential scanning calorimetry (DSC) was used, in conjunction with two functional assays that monitor binding, in a storage stability study on a protein of pharmaceutical interest, the soluble form of the T-lymphocyte multidomain surface receptor, sCD4. DSC monitored structural changes in binding and non-binding domains. ELISA, using the monoclonal antibody OKT4a, and frontal elution affinity chromatography, using the HIV surface glycoprotein, gp120, monitored function of the binding domain. The stability of sCD4 in a solution formulation was followed for up to 30 days at five differentpHs ranging from 5.0 to 7.9 and five different temperatures ranging from −70‡C to 40‡C. While the overall trends observed with the three techniques were the same, the ELISA data were somewhat less reproducible than those for the other methods. Furthermore, the results suggest that DSC is more sensitive to structural changes that would reduce the protein's bioactivity. The results of this study indicate DSC's utility, in conjunction with quantitative functional analysis, in the formulation of protein-containing pharmaceuticals or foods, especially those containing multiple-domain proteins.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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