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  • 1 Hebrew University-Hadassah Medical School Department of Biochemistry P.O. Box 12272 91120 Jerusalem Israel
  • | 2 Utrecht University for Pharmaceutical Sciences (UIPS) participant in the research school Groningen — Utrecht Institute for Drug Exploration (GUIDE), Faculty of Pharmacy, Utrecht University Department of Pharmaceutics Utrecht The Netherlands
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Abstract  

This study was aimed to investigate the physicochemical changes induced in 200 nm extruded oligolamellar DPPC:DPPG (10:1) liposomes by freezing, followed by γ-irradiation, in the absence and presence of 5 mM stable cyclic nitroxide radicals, 2,2,6,6-tetramethylpiperidine-1-oxyl (Tempo) and 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (Tempol). The characterization is based on the use of differential scanning calorimetry (DSC) and was aimed to differentiate the contribution of freezing and γ-irradiation in the presence and absence of nitroxides. Liposomal preparations of DPPC/DPPG which have sub-, pre- and main-phase transitions in the temperature range (0�C<Tm<50�C) were used. Our results show that: (1) freezing modified and induced fusion to MLV as well as fission to SUV, (2) freezing did not fully prevent the radiation-induced changes in the thermotropic characteristics of the liposomes, and (3) Tempo and Tempol did not prevent the changes in thermotropic behavior caused as a result of freezing of the liposomal dispersion. These results demonstrate that DSC is a powerful and sensitive tool in both physical and chemical studies of lipid assemblies.

Manuscript Submission: HERE

  • Impact Factor (2019): 2.731
  • Scimago Journal Rank (2019): 0.415
  • SJR Hirsch-Index (2019): 87
  • SJR Quartile Score (2019): Q3 Condensed Matter Physics
  • SJR Quartile Score (2019): Q3 Physical and Theoretical Chemistry
  • Impact Factor (2018): 2.471
  • Scimago Journal Rank (2018): 0.634
  • SJR Hirsch-Index (2018): 78
  • SJR Quartile Score (2018): Q2 Condensed Matter Physics
  • SJR Quartile Score (2018): Q2 Physical and Theoretical Chemistry

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
4
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)