Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 59: Issue 3
Yeast Intracellular Water Determination by Thermogravimetry
Authors:
E. Alcázar Universidade Federal do Rio de Janeiro Escola de Química Bl. E do CT – Cidade Universitária Rio de Janeiro RJ-21900 Brazil Bl. E do CT – Cidade Universitária Rio de Janeiro RJ-21900 Brazil

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M. Rocha-Leăo Universidade Federal do Rio de Janeiro Escola de Química Bl. E do CT – Cidade Universitária Rio de Janeiro RJ-21900 Brazil Bl. E do CT – Cidade Universitária Rio de Janeiro RJ-21900 Brazil

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J. Dweck Universidade Federal do Rio de Janeiro Escola de Química Bl. E do CT – Cidade Universitária Rio de Janeiro RJ-21900 Brazil Bl. E do CT – Cidade Universitária Rio de Janeiro RJ-21900 Brazil

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Pages:
643–648
Online Publication Date:
02 Nov 2004
Publication Date:
01 Mar 2000
Article Category:
Research Article
DOI:
https://doi.org/10.1023/a:1010172830355
Restricted access

Abstract  

The intracellular water content of a microorganism is an important parameter which is a determinant factor of its physiological properties. It is usually measured by complex and time consuming procedures. Thermogravimetry using infrared balance has been used for this purpose, through the identification of different drying steps occurring during the analysis. This work employs the same method with much smaller samples, using conventional thermogravimetric equipment in a simpler and faster way than other conventional procedures. Commercial yeast (Saccharomyces cerevisiae ) washed samples are analyzed in isothermal procedures which are run in about 30 min. The drying rate curve, when plotted as a function of the residual mass of the cells, allows the identification of the step where the intracellular water is lost and the determination of its content. The obtained values, on extracellular water free basis, are in the range of 65 to 69% and agree with those measured by other techniques.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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