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  • 1 TU Bergakademie Freiberg Institute of Physical Chemistry Leipziger Strasse 29 D-9596 Freiberg Germany
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Abstract  

This study furnished results on the enzymatic detection of phenolic compounds by means of a miniaturized heat-flow calorimeter (IC-calorimeter). Two enzymes were used: tyrosinase and peroxidase. Additionally to the investigations with the IC-calorimeter, measurements were carried out with a classical reaction calorimeter (LKB 8700) for the very slow reactions with tyrosinase. By way of contrast, the reactions with peroxidase are fast and seem more suitable for sensor application. The detection limit for the investigated phenolic compounds is of the order of 1 mmol l−1 .