Differential scanning calorimetry and high angle X-ray diffraction analyses were performed on gelatin films, air dried at
different values of constant elongation, crosslinked with glutaraldehyde and examined at constant relative humidity of 75%.
Drawing induces a preferential orientation of the chain segments of gelatin parallel to the stretching direction,and a linear
increase of the renaturation level, calculated as the ratio between the denaturationenthalpy of gelatin films and that of
tendon collagen. The comparison with the results previously obtained on the mechanical properties of the films, puts into
evidence the different contributions of orientation and renaturation on the improvement of the mechanical parameters on drawing.
The results offer important information on the role of glutaraldehyde (GTA) crosslinking on the stability of collagenous materials.