Authors:
M. Lu Peking University Institute of Physical Chemistry Beijing 100871 P. R. China

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B. Wang Peking University Institute of Physical Chemistry Beijing 100871 P. R. China

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Zh. Li Peking University Institute of Physical Chemistry Beijing 100871 P. R. China

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Y. Fei Chinese Academy of Sciences Institute of Developmental Biology Beijing 100080 P. R. China

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L. Wei Chinese Academy of Sciences Institute of Developmental Biology Beijing 100080 P. R. China

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Sh. Gao Chinese Academy of Sciences Institute of Developmental Biology Beijing 100080 P. R. China

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Abstract  

Antifreeze protein (AFP) can lower the freezing point by inhibiting the growth of ice crystals. In this article, the thermal hysteresis activity (THA) of a plant AFP was measured with differential scanning calorimetry (DSC). As is shown, when the amount of ice in the sample was less than 5% THA of this AFP reached as high as 0.35°C. The secondary structure of this AFP was studied with circular dichroism (CD). The CD spectrum from 195to 240 nm indicated a well-defined secondary structure consisting 11% α-helix, 34%antiparallel β-sheet and 55% random coil.

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation
1969
Volumes
per Year
1
Issues
per Year
24
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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