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  • 1 Huazhong Agricultural University Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry Wuhan 430070 P. R. China Wuhan 430070 P. R. China
  • 2 Wuhan University Department of Chemistry, College of Chemistry and Molecular Science Wuhan 430072 P. R. China Wuhan 430072 P. R. China
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Abstract  

A microcalorimetric technique based on the bacterial heat-output was explored to evaluate the effect of Mn(II) on Bacillus thuringiensis. The power-time curves of the growth metabolism of B. thuringiensis and the effect of Mn(II) on it were studied using an LKB-2277 BioActivity Monitor, ampoules method, at 28C. For evaluation of the results, the maximum peak-heat output power (Pmax) in the growth phase, the growth rate constants (k), the log phase heat effects (Qlog ), and the total heat effect in 23 h (QT) for B. thuringiensis were determined. Manganese has been regarded as the essential biological trace element. Mn(II) of different concentration have different effects on B. thuringiensis growth metabolism. High concentration (800-1600 μg mL-1) of Mn(II) can promote the growth of B. thuringiensis; low concentration (500-800 μg mL-1) can inhabit its growth.