Microcalorimetric Studies on the Product Inhibition of Hydrolysis of L-arginine Catalyzed by Bovine Liver Arginase

X. Xie; C. Wang; Z. Wang

doi:10.1023/b:jtan.0000027826.56859.04

Jump to Content Jump to Main Navigation

Journal of Thermal Analysis and Calorimetry

Volume/Issue:: Volume 76: Issue 1

Microcalorimetric Studies on the Product Inhibition of Hydrolysis of L-arginine Catalyzed by Bovine Liver Arginase

Authors: X. Xie¹, C. Wang¹, and Z. Wang¹

View More View Less

¹ Wuhan University College of Chemistry and Molecular Science Wuhan 430072 Hubei, People's Republic of China E-mail Wuhan 430072 Hubei, People's Republic of China E-mail

DOI:: https://doi.org/10.1023/b:jtan.0000027826.56859.04

Page Count:: 275–284

Publication Date:: 01 Apr 2004

Online Publication Date:: 03 Nov 2004

Article Category:: Research Article

Restricted access

Check for updates

Abstract

A new thermokinetic reduced extent method for the product inhibition of single substrate enzyme-catalyzed reactions is proposed and compared with the traditional initial rate method in this paper. The arginase-catalyzed hydrolysis of L-arginine to L-ornithine and urea was studied at 37C in 40 mM sodium barbiturate-HCl buffer solution (pH=9.4). Michaelis constant (K_m) for arginine and maximum velocity (V_m) of the reaction were determined by initial method and thermokinetic method. The activation of exogenous manganese to this reaction was also studied. The product inhibition constant (K_P), which cannot be obtained directly from the initial rate method, was determined by thermokinetic without adding L-ornithine to the reaction system. When the concentration of Mn²⁺ in cell is 0.1 mM, the enzyme gets its full activity. Incubation arginase with appropriate concentration of Mn²⁺resulted in increased Vmax and a higher sensitivity of the enzyme to product with no change in the K_m for arginine. We suggest that the exogenous manganese ions in solution have just recovered the activity of arginase, which was lost in dissolving and dilution, but no effect on the mechanism of the reaction.