Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 82: Issue 1
Calorimetric determination of enthalpies of lysozyme folding at a liquid-solid interface
Authors:
X. P. Geng Xi'an University of Engineering Science & Technology, College of Environment & Chemical Engineering Xi'an 710048, China, Xi'an 710048, China,

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H. F. Zhang Xi'an University of Engineering Science & Technology, College of Environment & Chemical Engineering Xi'an 710048, China Xi'an 710048, China

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B. H. Wang Institute of Physical Chemistry, Peaking University Beijing 100871, China Beijing 100871, China

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X. D. Geng Institute of Modern Separation Science, Northwest University Xi'an 710069, China Xi'an 710069, China

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J. W. Xing Xi'an University of Engineering Science & Technology, College of Textile Engineering & Material Xi'an 710048, China Xi'an 710048, China

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Pages:
193–199
Online Publication Date:
11 Jan 2006
Publication Date:
01 Sep 2005
Article Category:
Research Article
DOI:
https://doi.org/10.1007/s10973-005-0865-5
Keywords:
liquid-solid interface; lysozyme; micro-calorimetry; protein folding
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Summary  

Calorimetric determination of the total enthalpy changes (ΔHi) of guanidine-denatured lysozyme (Lys) during the adsorption with simultaneously refolding on the surface of hydrophobic interaction chromatography packings was carried out at 250.001C. The measured ΔHiin the circumstances should include the changes in the three fractions: adsorption, dehydration and molecular conformation. It was found that when the unfolded Lys molecules are adsorbed and refold on the surface, entropy-driving caused by the dehydration of Lys mainly dominates the foregoing process. The refolding enthalpies of Lys, ΔΔHiwere found to be 10~100 folds higher than that measured in usual solutions.</o:p>

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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