Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 83: Issue 1
A microcalorimetric study of the action of mercuric chloride on the metabolism of mitochondria isolated from Cyprinus carpio liver tissue
Authors:
J Zhu College of Chemistry and Molecular Sciences, Wuhan University Department of Chemical Biology Wuhan P. R. China 430072 Wuhan P. R. China 430072

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C Li Wuhan University School of Stomatology Wuhan P. R. China 430072 Wuhan P. R. China 430072

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Y Liu Wuhan University State Key Laboratory of Virology Wuhan P. R. China 430072 Wuhan P. R. China 430072

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Z Zhang College of Chemistry and Molecular Sciences, Wuhan University Department of Chemical Biology Wuhan P. R. China 430072 Wuhan P. R. China 430072

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A Hou College of Chemistry and Molecular Sciences, Wuhan University Department of Chemical Biology Wuhan P. R. China 430072 Wuhan P. R. China 430072

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S Qu College of Chemistry and Molecular Sciences, Wuhan University Department of Chemical Biology Wuhan P. R. China 430072 Wuhan P. R. China 430072

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Pages:
181–186
Online Publication Date:
19 Sep 2005
Publication Date:
08 Jan 2006
Article Category:
Research Article
DOI:
https://doi.org/10.1007/s10973-005-7020-1
Keywords:
Cyprinus carpio; mercuric chloride; microcalorimetry; mitochondria
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Abstract  

After the occurrence of 'Minamata disease' in 1950, mercury aroused much more attention, and lots of studies concerned have been made. The purpose of the present paper is to study the effect of mercuric chloride on the mitochondria suspension isolated from the liver tissue of Cyprinus carpio from the direct viewpoint of energy by using the microcalorimetric method. The metabolic thermogenic curves of the mitochondria suspension at 25C were obtained, and the mitochondria metabolic thermokinetic equations were established, from which we obtained the thermodynamic and thermokinetic parameters: thermogenic rate constant (k), heat output (Q), average heat power (Pav), etc. Experimental results indicated that low concentration of mercuric chloride (5 nmol Hg2+/(mg protein)) stimulates the thermogenesis of mitochondria, suggesting a strong effect of uncoupling action, while high concentration of mercuric chloride (20 nmol Hg2+/(mg protein)) inhibits the metabolism of mitochondria completely, suggesting a fatal effect on the phosphorylation system. The effect of Hg2+ on mitochondria is concentration-depended, from which the probable reaction mechanism of Hg2+ to the mitochondria was proposed. So the microcalorimetric method can be used in the toxicology research.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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