Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 83: Issue 3
Thermochemical, volumetric and spectroscopic properties of lysozyme–poly(ethylene) glycol system
Authors:
W Zielenkiewicz Institute of Physical Chemistry of the Polish Academy of Sciences Warsaw Poland 01-224 Kasprzaka 44/52 Warsaw Poland 01-224 Kasprzaka 44/52

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R Swierzewski Institute of Physical Chemistry of the Polish Academy of Sciences Warsaw Poland 01-224 Kasprzaka 44/52 Warsaw Poland 01-224 Kasprzaka 44/52

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F Attanasio CNR Institute for Macromolecular Studies – Division of Genova Genova Italy 16-149 Via De Marini 6 Genova Italy 16-149 Via De Marini 6

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G Rialdi CNR Institute for Macromolecular Studies – Division of Genova Genova Italy 16-149 Via De Marini 6 Genova Italy 16-149 Via De Marini 6

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Pages:
587–595
Online Publication Date:
07 Apr 2006
Publication Date:
12 Mar 2006
Article Category:
Research Article
DOI:
https://doi.org/10.1007/s10973-005-7417-x
Keywords:
density; DSC; fluorescence intensity; lysozyme unfolding; macromolecular crowding; PEG; preferential hydration
Restricted access

Abstract  

Investigations of lysozyme–polyethylene glycol system were made by differential scanning calorimetry, fluorescence and density techniques. The values of unfolding enthalpies, ΔHNU, unfolding temperatures, Tm, excess molar heat capacities, ΔCp, and apparent molar volumes, VΦ , were determined as functions of PEG concentration. The three PEGs of average molecular mass (MW) 6000, 10000, 20000 were used as macromolecular crowding agents. The concentration of polymers was changed in the range 0–30% mass per volume (w/v). The values of ΔHNU remained constant with no dependence on PEG concentration, while PEG addition to buffered lysozyme solutions caused linear decrease of Tm. The values of ΔCp and VΦ of lysozyme dramatically changed in the range of 8–10% of PEG concentration. The fluorescence spectroscopy was used in order to investigate the polymer influence on possible solvent–lysozyme interactions. The electrical properties of polymer–water and polymer–buffer systems, the dielectric constants of solutions were determined with use of impedance spectroscopy.

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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