was used to study the influence of NaCl concentration on Halobacterium
salinarum growth. From the thermogenic curves and thermokinetic
parameters of H. salinarum growth in different
concentrations of NaCl, it was found that the optimum NaCl concentration for H. salinarum growth was not a wide range from 3.5
mol L–1 to NaCl saturation (about 5.2 mol
L–1), as is generally acknowledged, but just
around 230 g L–1 (approximately 3.9 mol L–1).
And when external NaCl concentration was above 230 g L–1,
the growth metabolism of H. salinarum decreased
constantly with the increasing of NaCl concentration. These have never been
described before. Further investigation by transmission electron microscopy
revealed that H. salinarum growing in approaching
NaCl saturation underwent plasmolysis, which interpreted the novel finding
of microcalorimetry perfectly. Our work shows that microcalorimetry may reveal
more and newer details about microbial growth than the existing methods do.
These details are significant to understand biological processes.