Journal of Thermal Analysis and Calorimetry
Volume/Issue:
Volume 87: Issue 2
Interaction of a homologous series of n-alkyl trimethyl ammonium bromides with eggwhite lysozyme
Microcalorimetric and spectroscopic study
Authors:
A. K. Bordbar Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, Isfahan, Iran, 81746-73441

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R. Hosseinzadeh Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, Isfahan, Iran, 81746-73441
Food and Chemical Analysis Research Laboratory, Jahad-E-Daneshgahi, Urmia University, Urmia, Iran

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M. H. Norozi Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, Isfahan, Iran, 81746-73441

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Pages:
453–456
Online Publication Date:
07 Feb 2007
Publication Date:
01 Jan 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1007/s10973-005-6906-2
Keywords:
absorption spectroscopy; cationic surfactants; fluorescence spectroscopy; ITC; lysozyme; unfolding
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Abstract

The interaction of a series of n-alkyl trimethyl ammonium bromides (C12, C14 and C16) with egg white lysozyme have been studied using fluorescence and UV-Vis spectroscopies and isothermal titration calorimetry (ITC). The trend of variation of molar absorptivity at 281 nm, quantum yields (λex=281 nm) and heat of interaction with respect to surfactant concentration, were measured. The spectrophotometric results show that the hydrophobic interactions have a major role in denaturation mechanism and it would be increased with increasing in hydrocarbon tail length of surfactant. The ITC results indicated the two-step mechanism for unfolding of lysozyme due to its interaction with surfactants.

  • 1 Chodankar, S Aswal, VK 2005 Phys. Rev. E 72 041931 .

  • 2 Manetto, GD La Rosa, C Grasso, DM Milardi, D 2005 J. Therm. Anal. Cal. 80 263 .

  • 3 Michnik, A Michalik, K Drzazga, Z 2005 J. Therm. Anal. Cal. 80 399 .

  • 4 Pace, CN 1990 Trends Biotechnol. 8 93 .

  • 5 Vieille, C Zeikus, JG 1996 Trends Biotechnol. 14 183 .

  • 6 Jones, M. N. and Manley, P., Surfactants Solution, Proc. Int. Symp., 4th (1984) Meeting Date 1982, pp. 2, 1403.

  • 7 Imoto, T Sami, S Tsuru, M Yagishita, K 1979 Agric. Biol. Chem. 43 1809 .

  • 8 Hayashi, K Kugimiya, M Imoto, T Funatsu, M Bigelow, CC 1968 Biochemistry 7 1461 .

  • 9 Hayashi, K Kugimiya, M Imoto, T Funatsu, M Bigelow, CC 1968 Biochemistry 7 1467 .

  • 10 Subramanian, M Sheshadri, BS Venkatappa, MP 1984 J. Biochem. 95 413.

  • 11 Saboury, AA 2003 J. Therm. Anal. Cal. 72 93 .

  • 12 Waters, LJ Leharna, SA Mitchell, JC 2005 J. Therm. Anal. Cal. 80 43 .

  • 13 de Rivera, MR Socorro, F 2005 J. Therm. Anal. Cal. 80 769 .

  • 14 Sophianopoulos, AJ Khodes, CK Holcomb, DN Holde, KE 1962 J. Biol. Chem. 237 1107.

  • 15 Kresheck, GC Hargraves, WA 1974 J. Colloid Interface Sci. 48 481 .

  • 16 Kresheck, GC 1998 J. Phys. Chem. B 102 6596 .

  • 17 Olofsson, G 1985 J. Phys. Chem. 89 1473 .

  • 18 Johnson, I Olofsson, G Jonsson, B 1987 J. Chem. Soc. Faraday Trans. 183 3331.

  • 19 Paula, S Sus, W Tuchtenhagen, J Blume, A 1995 J. Phys. Chem. 99 11742 .

  • 20 Rosen, MJ et al. 1989 ‘Surfactants and Interfacial Phenomena’ Surfactant Research Institute Brooklyn College Chapter 3 p. 122.

  • 21 Saboury, AA Bordbar, AK Moosavi-Movahedi, AA 1996 J. Chem. Thermodyn. 28 1077 .

  • 22 Saboury, AA Moosavi-Movahedi, AA 1996 Bull. Chem. Soc. Jpn. 69 3031 .

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Cover Journal of Thermal Analysis and Calorimetry
Journal of Thermal Analysis and Calorimetry
Print ISSN:
1388-6150
Online ISSN:
1572-8943

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Journal of Thermal Analysis and Calorimetry
Language English
Size A4
Year of
Foundation		 1969
Volumes
per Year		 1
Issues
per Year		 24
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 1388-6150 (Print)
ISSN 1588-2926 (Online)

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