Authors: L. Ruan, Y. Wang, L. Wai and Y. Hoi-fu
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  • 1 State Key Laboratory of Chinese Medicine and Molecular Pharmacology Shenzhen China
  • 2 Hong Kong Polytechnic University Applied Biology and Chemical Technology Hong Kong China
  • 3 Huazhong Agricultural University State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology Wuhan 430070 China
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Abstract  

The thermogenic curves of metabolism of the four strains of Escherichia coli pUC19cab/JM109, pUC19cab/XL-IBlue, JM109 and XL-IBlue were determined using the LKB-2277 BioActivity Monitor and the ampoule method at 37�C. The pUC19cab/JM109 and pUC19cab/XL-IBlue are recombinant E. coli strains bearing the same foreign plasmid pUC19cab, which confers the ability to produce polyhydroxyalkanoates (PHAs). The yield of PHAs of pUC19cab/XL-IBlue was higher than that of pUC19cab/JM109. XL-IBlue and JM109 were the host bacteria. The heat flow of these strains was XL-Blue≈JM109>pUC19cab/JM109>pUC19cab/XL-IBlue. These results indicate an obvious interrelation between the PHAs production and the heat flow rate of E. coli strains.