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  • 1 Laurentian University Department of Biology Sudbury ON P3E 2C6 Canada
  • 2 Laurentian University Department of Chemical Engineering Sudbury ON P3E 2C6 Canada
  • 3 University of Waterloo Dept. of Chemical Engineering 200 University Ave. West Waterloo ON N2L 3G1 Canada
  • 4 University of New Brunswick Saint John NB E2L 4L5 Canada
  • 5 University of Toronto Centre for Biocomposites and Biomaterials Processing, Faculty of Forestry 33 Willcocks St. Toronto ON M5S 3B3 Canada
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Abstract  

One of the limiting factors to the effectiveness of biostimulation and bioremediation is the loss of inoculated material from the site. This can occur by a number of pathways, but is particularly problematic in open water systems where the inoculated material is simply lost in the water. It is desirable to develop new material, a matrix, within which bacteria and/or biostimulants can be incorporated. We have investigated the basic physical properties of insoluble potato starch to eventually evaluate its use as such a matrix. Insoluble starch fibers were prepared from white potato (Solanum tuberosum) and sweet potato (Ipomoea batatas) and were compared for their melting temperature by DSC and their ability to bind/aggregate bacteria. The DSC curves for white and sweet potato showed that the melting temperature is 127.34 and 133.05�C for white and sweet potato fibers, respectively. The TG curves for white and sweet potato starches exhibited one main mass loss step corresponding to the DTG peak temperature at 323.39 and 346.93�C, respectively. The two types of fibers, however, showed different binding/aggregation capacities for bacteria, with white potato approximately twice as many cells of Burkholderia cepacia (22.6 billion/g) as cells of Pseudomonas putida. The reverse was true for fibers from sweet potato, binding twice as many cells of Pseudomonas putida (23 billion/g) as cells of Burkholderia cepacia.

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